含有大肠杆菌B/r的araBAD基因的杂种质粒。

Hybrid plasmids containing the araBAD genes of Escherichia coli B/r.

作者信息

Kaplan D A, Greenfield L, Boone T, Wilcox G

出版信息

Gene. 1978 May;3(3):177-89. doi: 10.1016/0378-1119(78)90031-8.

Abstract

The DNA fragments generated by restriction endonuclease BamI which contain the araCBAD genes from E.coli B/r have been cloned. The DNA fragments containing ara genes were idenified by a compairson of the BamI fragments of lambdah80dara phages containing different ara deletion mutations. The ara genes were cloned into the plasmid pBR317, a derivative of ColE1. The cloned DNA fragments were analyzed by digestion with pairs of restriction endonucleases to determine the molecular weight of the chimeras and to identify the cloned ara DNA fragments. The cloned ara fragments were also identified by genetic complementation and recombination tests.

摘要

由限制性内切酶BamI产生的含有来自大肠杆菌B/r的araCBAD基因的DNA片段已被克隆。通过比较含有不同ara缺失突变的λh80dara噬菌体的BamI片段，鉴定出了含有ara基因的DNA片段。将ara基因克隆到质粒pBR317（ColE1的衍生物）中。通过用一对限制性内切酶消化来分析克隆的DNA片段，以确定嵌合体的分子量并鉴定克隆的ara DNA片段。还通过遗传互补和重组试验鉴定了克隆的ara片段。