Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.

Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.