噬菌体T7与T7 DNA克隆片段之间的基因重组和互补作用。
Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA.
作者信息
Campbell J L, Richardson C C, Studier F W
出版信息
Proc Natl Acad Sci U S A. 1978 May;75(5):2276-80. doi: 10.1073/pnas.75.5.2276.
Abstract
Frafments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.
摘要
利用质粒pMB9已将噬菌体T7 DNA片段克隆到大肠杆菌中。这种克隆片段能够与感染性噬菌体发生重组，从而提供了一种整合T7 DNA物理图谱和遗传图谱的方法。到目前为止，在克隆中已发现约65%的T7 DNA分子，对这些克隆的分析已将基因12 - 17定位，其精度约为T7 DNA全长的1%。至少一些克隆片段能为感染性噬菌体提供T7功能。
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