Kammermeier H, Wendtland B
J Mol Cell Cardiol. 1987 Feb;19(2):167-75. doi: 10.1016/s0022-2828(87)80559-x.
Interstitial fluid (IF) emerging at the ventricular surface of isolated perfused rat hearts was collected and assayed for rate of production, protein content, glucose and lactate concentration. The influence of four perfusion media was investigated during an experimental period of 150 min: Krebs-Henseleit solution (KH) containing glucose (5.5 mM); KH containing glucose and pyruvate (2 mM); Hypoxic KH (20% O2), substrates as 2; KH containing isoprenaline (8 X 10(-9) M), substrates as 2. Interstitial fluid was produced at a rate of 20 to 100 microliters/min/gww and contained proteins (0.5 to 3 g/l). Interstitial glucose concentration was lower than venous concentration by up to 50%. Interstitial lactate concentration was higher by up to 600%. Permeability X surface area products of glucose calculated from transcapillary concentration differences and transfer rates were different depending on experimental conditions, but were within the rather large range of P X S values for molecules of similar size obtained by other authors. Those of lactate were higher by a factor of 3 to 9 and can be interpreted to be influenced by the metabolic activity of the endothelium. The results demonstrate that interstitial substrate concentration can differ very markedly from intravascular concentration and cannot be estimated reliably without assay of the interstitial fluid. Capillary permeability seems to be variable under the experimental conditions of this study.
收集分离灌注大鼠心脏心室表面出现的间质液（IF），并测定其生成速率、蛋白质含量、葡萄糖和乳酸浓度。在150分钟的实验期内，研究了四种灌注介质的影响：含葡萄糖（5.5 mM）的克雷布斯 - 亨泽莱特溶液（KH）；含葡萄糖和丙酮酸（2 mM）的KH；低氧KH（20% O₂），底物同2；含异丙肾上腺素（8×10⁻⁹ M）的KH，底物同2。间质液以20至100微升/分钟/克湿重的速率生成，含有蛋白质（0.5至3克/升）。间质葡萄糖浓度比静脉浓度低达50%。间质乳酸浓度比静脉浓度高600%。根据跨毛细血管浓度差和转运速率计算的葡萄糖通透性×表面积产物因实验条件而异，但在其他作者获得的类似大小分子的P×S值的相当大范围内。乳酸的通透性×表面积产物高3至9倍，可解释为受内皮细胞代谢活性的影响。结果表明，间质底物浓度可能与血管内浓度有非常显著的差异，未经间质液测定无法可靠估计。在本研究的实验条件下，毛细血管通透性似乎是可变的。
