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通过超声检查和母体血液转录组分析在孕期识别巨大儿综合征。

Identification of large offspring syndrome during pregnancy through ultrasonography and maternal blood transcriptome analyses.

机构信息

Division of Animal Sciences, University of Missouri, 164 ASRC, 920 East Campus Drive, Columbia, MO, 65211, USA.

Department of Animal and Food Sciences, Oklahoma State University, Stillwater, USA.

出版信息

Sci Rep. 2022 Jun 22;12(1):10540. doi: 10.1038/s41598-022-14597-w.

DOI:10.1038/s41598-022-14597-w
PMID:35732675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9217928/
Abstract

In vitro production (IVP) of embryos in cattle can result in large/abnormal offspring syndrome (LOS/AOS) which is characterized by macrosomia. LOS can cause dystocia and lead to the death of dam and calf. Currently, no test exists to identify LOS pregnancies. We hypothesized that fetal ultrasonography and/or maternal blood markers are useful to identify LOS. Bovine fetuses were generated by artificial insemination (control) or IVP. Fetal ultrasonographies were taken on gestation D55 (D55) and fetal collections performed on D56 or D105 (gestation in cattle ≈ D280). IVP fetuses weighing ≥ 97 percentile of the control weight were considered LOS. Ultrasonography results show that the product of six D55 measurements can be used to identify extreme cases of LOS. To determine whether maternal blood can be used to identify LOS, leukocyte mRNA from 23 females was sequenced. Unsupervised hierarchical clustering grouped the transcriptomes of the two females carrying the two largest LOS fetuses. Comparison of the leukocyte transcriptomes of these two females to the transcriptome of all other females identified several misregulated transcripts on gestation D55 and D105 with LOC783838 and PCDH1 being misregulated at both time-points. Together our data suggest that LOS is identifiable during pregnancy in cattle.

摘要

牛的体外胚胎生产（IVP）可能导致大型/异常后代综合征（LOS/AOS），其特征是巨大儿。LOS 可导致难产，并导致母畜和小牛死亡。目前，尚无检测方法可识别 LOS 妊娠。我们假设胎儿超声和/或母体血液标志物可用于识别 LOS。通过人工授精（对照）或 IVP 生成牛胎儿。在妊娠 D55 时进行胎儿超声检查（D55），并在 D56 或 D105 进行胎儿采集（牛的妊娠约为 D280）。体重超过对照体重 97 百分位的 IVP 胎儿被认为是 LOS。超声检查结果表明，六个 D55 测量值的乘积可用于识别 LOS 的极端情况。为了确定母体血液是否可用于识别 LOS，对 23 名女性的白细胞 mRNA 进行了测序。无监督层次聚类将携带两个最大 LOS 胎儿的两名女性的转录组分组。比较这两名携带最大 LOS 胎儿的女性的白细胞转录组与所有其他女性的转录组，确定了在妊娠 D55 和 D105 时几个失调的转录本，其中 LOC783838 和 PCDH1 在两个时间点均失调。总之，我们的数据表明，在牛的妊娠期间可以识别 LOS。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/620832bb2ea3/41598_2022_14597_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/5d9096dde277/41598_2022_14597_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/94a0d8582d29/41598_2022_14597_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/8ba5f750591a/41598_2022_14597_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/e6e19fdd4a3a/41598_2022_14597_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/a172b0600e23/41598_2022_14597_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/9d0a088adba7/41598_2022_14597_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/8fe6d8cf3e0f/41598_2022_14597_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/620832bb2ea3/41598_2022_14597_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/5d9096dde277/41598_2022_14597_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/94a0d8582d29/41598_2022_14597_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/8ba5f750591a/41598_2022_14597_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/e6e19fdd4a3a/41598_2022_14597_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/a172b0600e23/41598_2022_14597_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/9d0a088adba7/41598_2022_14597_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/8fe6d8cf3e0f/41598_2022_14597_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a34/9217928/620832bb2ea3/41598_2022_14597_Fig8_HTML.jpg

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