人诱导多能干细胞衍生的血脑屏障体外模型。
An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.
机构信息
Stroke Research Group, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
出版信息
Methods Mol Biol. 2022;2492:103-116. doi: 10.1007/978-1-0716-2289-6_5.
Human induced pluripotent stem cells (hiPSC) offer a tractable system to model the blood-brain barrier (BBB). Here we detail the assembly of a triple co-culture hiPSC-BBB model, using hiPSC-derived brain microvascular endothelial cells (BMEC), astrocytes, and mural cells (MC). Transendothelial electrical resistance (TEER) and sodium fluorescein (NaFl) permeability can be used to test the barrier properties. The model has applications in studying BBB-related pathology and for drug screening.
人诱导多能干细胞（hiPSC）为模拟血脑屏障（BBB）提供了一个易于处理的系统。本文详细介绍了三重共培养 hiPSC-BBB 模型的组装，使用 hiPSC 来源的脑微血管内皮细胞（BMEC）、星形胶质细胞和壁细胞（MC）。跨内皮电阻（TEER）和钠荧光素（NaFl）通透性可用于测试屏障特性。该模型可用于研究与 BBB 相关的病理学和药物筛选。
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