Department of Biology, Faculty of Science and Literature, Bolu Abant Izzet Baysal University, 14030, Bolu, Turkey.
Center for Innovative Food Technologies Development, Application and Research, Bolu Abant Izzet Baysal University, 14030, Bolu, Turkey.
Mol Biol Rep. 2022 Jul;49(7):7173-7183. doi: 10.1007/s11033-022-07634-4. Epub 2022 Jun 22.
Although members of the SDR gene family (short chain dehydrogenase) are distributed in kingdom of life, they have diverse roles in stress tolerance mechanism or secondary metabolite biosynthesis. Nevertheless, their precise roles in gene expression or regulation under stress are yet to be understood.
As a case study, we isolated, sequenced and functionally characterized the 3β-HSD promoter from Digitalis ferruginea subsp. ferruginea in Arabidopsis thaliana.
The promoter fragment contained light and stress response elements such as Box-4, G-Box, TCT-motif, LAMP element, ABRE, ARE, WUN-motif, MYB, MYC, W box, STRE and Box S. The functional analysis of the 3β-HSD promoter in transgenic Arabidopsis seedlings showed that the promoter was expressed in cotyledon and root elongation zone in 2 days' seedlings. However, this expression was extended to hypocotyl and complete root in 6 days' seedlings. In 20 days-old seedlings, promoter expression was distributed to the whole seedling including hydathodes aperture, vascular bundle, shoot apical meristem, trichomes, midrib, leaf primordia, hypocotyl and xylem tissues. Further, expression of the promoter was enhanced or remained stable under the different abiotic stress conditions like osmotic, heat, cold, cadmium or low pH. In addition, the promoter also showed response to methyl jasmonate (MeJA) application. The expression could not be induced in wounded cotyledon most likely due to lack of interacting elements in the promoter fragment.
Taken together, the 3β-HSD promoter could be a candidate for the development of transgenic plants especially under changing environmental conditions.
尽管 SDR 基因家族（短链脱氢酶）成员分布在生命王国中，但它们在应激耐受机制或次生代谢物生物合成中具有多种作用。然而，它们在应激下基因表达或调控中的精确作用仍有待了解。
作为一个案例研究，我们在拟南芥中分离、测序并功能表征了毛地黄亚种毛地黄的 3β-HSD 启动子。
启动子片段包含光和应激响应元件，如 Box-4、G-Box、TCT 基序、LAMP 元件、ABRE、ARE、WUN 基序、MYB、MYC、W 盒、STRE 和 Box S。3β-HSD 启动子在转基因拟南芥幼苗中的功能分析表明，启动子在 2 天龄幼苗的子叶和根伸长区表达。然而，这种表达在 6 天龄幼苗中延伸到下胚轴和完整的根。在 20 天龄的幼苗中，启动子的表达分布在整个幼苗中，包括水孔开口、维管束、茎尖分生组织、毛状体、中脉、叶原基、下胚轴和木质部组织。此外，启动子在不同的非生物胁迫条件下，如渗透、热、冷、镉或低 pH 下，表达增强或保持稳定。此外，启动子还对茉莉酸甲酯（MeJA）的应用有反应。在受伤的子叶中，由于启动子片段中缺乏相互作用元件，表达不能被诱导。
综上所述，3β-HSD 启动子可能是开发转基因植物的候选者，特别是在不断变化的环境条件下。
