Latif Sara, Sameeullah Muhammad, Abbasi Hiffza Qadeer, Masood Zainab, Demiral Sert Tijen, Aslam Noreen, Pekdemir Turgay, Imren Mustafa, Çiftçi Vahdettin, Saba Kiran, Malik Muhammad Suleman, Ijaz Fatima, Batool Neelam, Mirza Bushra, Waheed Mohammad Tahir
Department of Biology, University of Haripur, Haripur, Pakistan.
Department of Field Crops, Faculty of Agriculture, Bolu Abant Izzet Baysal University, Bolu, Türkiye.
Front Pharmacol. 2024 Dec 11;15:1421131. doi: 10.3389/fphar.2024.1421131. eCollection 2024.
The global prevalence of diabetes among adults over 18 years of age is expected to increase from 10.5% to 12.2% (between 2021 and 2045). Plants can be a cost-effective source of flavonoids like quercetin and kaempferol with anti-diabetic properties.
We aimed to assess the antidiabetic potential of leaves of cvs. Green Sprout and Marathon. Further, flavonoid contents were measured in broccoli leaves grown under light and dark conditions. The methanolic extracts of Green Sprout (GSL-M) and Marathon (ML-M) were first evaluated for their α-amylase and α-glucosidase inhibitory potential and then for antidiabetic activity in alloxan-induced diabetic rat models.
Treatment with plant extracts promoted the reduced glutathione (GSH) content and CAT, POD, and SOD activities in the pancreas, liver, kidney, heart, and brain of diabetic rats, whereas lowered lipid peroxidation, HO, and nitrite concentrations. The histopathological studies revealed the protective effect of plant extracts at high dose (300 mg/kg), which could be due to broccoli's rich content of chlorogenic acid, quercetin, and kaempferol. Strikingly, etiolated leaves of broccoli manifested higher levels of quercetin and kaempferol than green ones. The putative role of an ABC transporter in the accumulation of quercetin and kaempferol in etiolated leaves was observed as evaluated by qRT-PCR and analyses.
In conclusion, the present study shows a strong link between the antidiabetic potential of broccoli due to the presence of chlorogenic acid, quercetin, and kaempferol and the role of an ABC transporter in their accumulation within the vacuole.
预计18岁以上成年人中糖尿病的全球患病率将从10.5%增至12.2%（2021年至2045年期间）。植物可以成为具有抗糖尿病特性的黄酮类化合物（如槲皮素和山奈酚）的经济有效来源。
我们旨在评估绿芽品种和马拉松品种叶片的抗糖尿病潜力。此外，还测定了在光照和黑暗条件下生长的西兰花叶片中的黄酮类化合物含量。首先评估绿芽品种（GSL-M）和马拉松品种（ML-M）的甲醇提取物对α-淀粉酶和α-葡萄糖苷酶的抑制潜力，然后在四氧嘧啶诱导的糖尿病大鼠模型中评估其抗糖尿病活性。
用植物提取物处理可提高糖尿病大鼠胰腺、肝脏、肾脏、心脏和大脑中的还原型谷胱甘肽（GSH）含量以及CAT、POD和SOD活性，同时降低脂质过氧化、HO和亚硝酸盐浓度。组织病理学研究显示高剂量（300 mg/kg）植物提取物具有保护作用，这可能归因于西兰花富含绿原酸、槲皮素和山奈酚。令人惊讶的是，西兰花的黄化叶中槲皮素和山奈酚的含量高于绿叶。通过qRT-PCR和分析评估发现，ABC转运蛋白在黄化叶中槲皮素和山奈酚积累中发挥了推定作用。
总之，本研究表明西兰花因含有绿原酸、槲皮素和山奈酚而具有的抗糖尿病潜力与ABC转运蛋白在其液泡内积累中的作用之间存在紧密联系。
