Yan Huiling, Zhang Xinxin, Li Xiang, Wang Xuelai, Li Hanxi, Zhao Qiushuang, Yin Peng, Guo Ruixue, Pei Xiaona, Hu Xiaoqing, Han Rui, Zhao Xiyang
College of Forestry and Grassland, Jilin Agricultural University, Changchun, China.
Front Bioeng Biotechnol. 2022 Jun 6;10:911701. doi: 10.3389/fbioe.2022.911701. eCollection 2022.
× (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, () was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentation leaves. The transcriptome analysis between wild type (WT) and AM showed that 170 differentially expressed genes (DEGs) (86 up-regulated and 84 down-regulated) were found, and some DEGs were involved in flavone and flavonol biosynthesis, flavonoid biosynthesis and anthocyanin biosynthesis. The metabolome analysis showed that 13 anthocyanins-related differentially accumulated metabolites (DAMs) were detected in AM. The correlation analysis between DEGs and DAMs were performed, and the results revealed that 18 DEGs, including 11 MYB genes, two genes, one gene, one gene, and three genes, were negatively or positively correlated with 13 DAMs. The phylogenetic analysis demonstrated that there was high homology between and , and MYB113 co-expressed with BZ1, ANS and DFR directly. Our results elucidated the molecular mechanism of plant color change mediated by anthocyanin biosynthesis pathway, which laid the foundation for the development and utilization of colorful woody plant.
×（84K杨树）是具有优异基因工程资源和观赏价值的模式材料。在我们的研究中，（某基因）在84K杨树中过表达，并且花青素含量高的转基因84K（AM）杨树表现出叶片呈红色色素沉着。野生型（WT）和AM之间的转录组分析表明，发现了170个差异表达基因（DEGs）（86个上调和84个下调），并且一些DEGs参与黄酮和黄酮醇生物合成、类黄酮生物合成和花青素生物合成。代谢组分析表明，在AM中检测到13种与花青素相关的差异积累代谢物（DAMs）。进行了DEGs和DAMs之间的相关性分析，结果显示18个DEGs，包括11个MYB基因、2个（其他基因）、1个（其他基因）、1个（其他基因）和3个（其他基因），与13个DAMs呈负相关或正相关。系统发育分析表明（某基因）和（另一基因）之间具有高度同源性，并且MYB113与BZ1、ANS和DFR直接共表达。我们的结果阐明了由花青素生物合成途径介导的植物颜色变化的分子机制，这为彩色木本植物的开发利用奠定了基础。
