Irani Mohammad, Nodeh Sina Mohammadrezaei
Faculty of Pharmacy, Alborz University of Medical Sciences Karaj Iran
RSC Adv. 2022 Jun 1;12(25):16310-16318. doi: 10.1039/d2ra02150a. eCollection 2022 May 23.
Gold nanoparticles, paclitaxel (PTX), and camptothecin (CMPT) were loaded into the PVA/κ-carrageenan/pegylated-PU composite and core-shell nanofibers prepared by two-nozzle and coaxial electrospinning methods. The capability of composite and core-shell nanofibers was investigated for the targeted delivery of anticancer drugs in lung cancer treatment. and release of PTX and CMPT were investigated to find the release mechanism from nanofibers compared to direct administration of pristine PTX and CMPT. The mean fiber diameter for composite and core-shell nanofibers with shell feeding rates of 0.3, 0.5, and 0.7 mL h was about 225, 330, 520, and 640 nm, respectively. release studies indicated that the blood concentration of CMPT and PTX for rats fed with core-shell nanofibers reached the highest values of 26.8 ± 0.04 μg mL, and 26.5 ± 0.05 μg mL in 36 h, and 24 h and reduced slowly within 84 h, and 48 h, respectively. The maximum cytotoxicity was 75% in the presence PVA/κ-carrageenan/CMPT/Au/pegylated-PU/PTX core-shell nanofibers. antitumor activity results confirmed the synergic effect of Au, CMPT and PTX anticancer drugs on the reduction of tumor volume without change in mouse weight by the PVA/κ-carrageenan/CMPT/Au/pegylated PU/PTX core-shell nanofibers. The obtained results indicated that the simultaneous loading of CMPT and PTX anticancer drugs and Au nanoparticles is more beneficial for lung cancer treatment.
将金纳米颗粒、紫杉醇（PTX）和喜树碱（CMPT）负载到通过双喷嘴和同轴静电纺丝法制备的PVA/κ-卡拉胶/聚乙二醇化聚氨酯复合核壳纳米纤维中。研究了复合核壳纳米纤维在肺癌治疗中靶向递送抗癌药物的能力。研究了PTX和CMPT的释放情况，以找出与直接施用原始PTX和CMPT相比，纳米纤维的释放机制。壳进料速率为0.3、0.5和0.7 mL/h时，复合核壳纳米纤维的平均纤维直径分别约为225、330、520和640 nm。释放研究表明，喂食核壳纳米纤维的大鼠体内CMPT和PTX的血药浓度分别在36 h和24 h达到最高值，分别为26.8±0.04 μg/mL和26.5±0.05 μg/mL，并在84 h和48 h内缓慢下降。在PVA/κ-卡拉胶/CMPT/Au/聚乙二醇化聚氨酯/PTX核壳纳米纤维存在下，最大细胞毒性为75%。抗肿瘤活性结果证实了Au、CMPT和PTX抗癌药物通过PVA/κ-卡拉胶/CMPT/Au/聚乙二醇化聚氨酯/PTX核壳纳米纤维对减少肿瘤体积具有协同作用，且小鼠体重无变化。所得结果表明，同时负载CMPT和PTX抗癌药物以及Au纳米颗粒对肺癌治疗更有益。
