Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
Nat Prod Rep. 2022 Jul 20;39(7):1510-1530. doi: 10.1039/d2np00011c.
Covering: 2017 to 2022Mass spectrometry imaging (MSI) has become a mature molecular imaging technique that is well-matched for natural product (NP) discovery. Here we present a brief overview of MSI, followed by a thorough discussion of different MSI applications in NP research. This review will mainly focus on the recent progress of MSI in plants and microorganisms as they are the main producers of NPs. Specifically, the opportunity and potential of combining MSI with other imaging modalities and stable isotope labeling are discussed. Throughout, we focus on both the strengths and weaknesses of MSI, with an eye on future improvements that are necessary for the progression of MSI toward routine NP studies. Finally, we discuss new areas of research, future perspectives, and the overall direction that the field may take in the years to come.
2017 年至 2022 年
质谱成像（MSI）已成为一种成熟的分子成像技术，非常适合天然产物（NP）的发现。在这里，我们简要介绍了 MSI，随后深入讨论了 MSI 在 NP 研究中的不同应用。本综述将主要关注植物和微生物中 MSI 的最新进展，因为它们是 NP 的主要生产者。具体来说，我们讨论了将 MSI 与其他成像模式和稳定同位素标记相结合的机会和潜力。整篇文章都关注 MSI 的优缺点，并着眼于未来的改进，这对于 MSI 向常规 NP 研究的发展是必要的。最后，我们讨论了新的研究领域、未来展望以及该领域在未来几年可能的发展方向。
