Kanyesigye Dalton, Alibu Vincent Pius, Tay Wee Tek, Nalela Polycarp, Paparu Pamela, Olaboro Samuel, Nkalubo Stanley Tamusange, Kayondo Ismail Siraj, Silva Gonçalo, Seal Susan E, Otim Michael Hilary
National Agricultural Research Organization (NARO), National Crops Resources Research Institute (NaCRRI), Kampala P.O. Box 7084, Uganda.
College of Veterinary Medicine, Animal Resources and Biosecurity (CoVAB), Makerere University, Kampala P.O. Box 7062, Uganda.
Insects. 2022 Jun 14;13(6):543. doi: 10.3390/insects13060543.
Bean leaf beetle (BLB) () has emerged as an important bean pest in Uganda, leading to devastating crop losses. There is limited information on the population genetic structure of BLB despite its importance. In this study, novel microsatellite DNA markers and the partial mitochondrial cytochrome oxidase subunit I (mt) gene sequences were used to analyze the spatial population genetic structure, genetic differentiation and haplotype diversity of 86 samples from 16 (districts) populations. We identified 19,356 simple sequence repeats (SSRs) (mono, di-, tri-, tetra-, penta-, and hexa-nucleotides) of which 81 di, tri and tetra-nucleotides were selected for primer synthesis. Five highly polymorphic SSR markers (4-21 alleles, heterozygosity 0.59-0.84, polymorphic information content (PIC) 50.13-83.14%) were used for this study. Analyses of the 16 populations with these five novel SSRs found nearly all the genetic variation occurring within populations and there was no evidence of genetic differentiation detected for both types of markers. Also, there was no evidence of isolation by distance between geographical and genetic distances for SSR data and mt data except in one agro-ecological zone for mt data. Bayesian clustering identified a signature of admixture that suggests genetic contributions from two hypothetical ancestral genetic lineages for both types of markers, and the minimum-spanning haplotype network showed low differentiation in minor haplotypes from the most common haplotype with the most common haplotype occurring in all the 16 districts. A lack of genetic differentiation indicates unrestricted migrations between populations. This information will contribute to the design of BLB control strategies.
豆叶甲（BLB）已成为乌干达一种重要的豆类害虫，导致了毁灭性的作物损失。尽管其很重要，但关于豆叶甲种群遗传结构的信息却很有限。在本研究中，利用新型微卫星DNA标记和部分线粒体细胞色素氧化酶亚基I（mt）基因序列，对来自16个地区种群的86个样本的空间种群遗传结构、遗传分化和单倍型多样性进行了分析。我们鉴定出19356个简单序列重复（SSR）（单核苷酸、二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸），从中选择了81个二核苷酸、三核苷酸和四核苷酸用于引物合成。本研究使用了5个高度多态性的SSR标记（4 - 21个等位基因，杂合度0.59 - 0.84，多态信息含量（PIC）50.13 - 83.14%）。用这5个新型SSR对16个种群进行分析发现，几乎所有遗传变异都发生在种群内部，两种标记均未检测到遗传分化的证据。此外，除了mt数据在一个农业生态区外，SSR数据和mt数据的地理距离与遗传距离之间均未发现距离隔离的证据。贝叶斯聚类识别出一种混合特征，表明两种标记均来自两个假定祖先遗传谱系的遗传贡献，最小生成单倍型网络显示，与所有16个地区都出现的最常见单倍型相比，次要单倍型的分化程度较低。缺乏遗传分化表明种群间存在不受限制的迁移。这些信息将有助于设计豆叶甲的控制策略。
