CSIRO, Black Mountain Laboratories, ACT, Australia.
CSIRO, Brisbane, Australia.
Pest Manag Sci. 2018 Jan;74(1):170-173. doi: 10.1002/ps.4676. Epub 2017 Sep 25.
The whitefly Bemisia tabaci complex harbours over 40 cryptic species that have been placed in 11 phylogenetically distinct clades based on the molecular characterization of partial mitochondrial DNA COI (mtCOI) gene region. Four cryptic species are currently within the invasive clade, i.e. MED, MEAM1, MEAM2 and IO. Correct identification of these species is a critical step towards implementing reliable measures for plant biosecurity and border protection; however, no standardized B. tabaci-specific primers are currently available which has caused inconsistencies in the species identification processes.
We report three sets of polymerase chain reaction (PCR) primers developed to amplify the mtCOI region which can be used for genotyping MED, MEAM1 and IO species, and tested these primers on 91 MED, 35 MEAM1 and five IO individuals. PCR and sequencing of amplicons identified a total of 21, six and one haplotypes in MED, MEAM1 and IO respectively, of which six haplotypes were new to the B. tabaci database.
These primer pairs enabled standardization and robust molecular species identification via mtCOI screening of the targeted invasive cryptic species and will improve quarantine decisions. Use of this diagnostic tool could be extended to other species within the complex. © 2017 Society of Chemical Industry.
烟粉虱复合种中包含超过 40 种隐种，这些隐种根据部分线粒体 DNA COI（mtCOI）基因区域的分子特征被划分为 11 个不同的系统发育分支。目前有 4 种隐种（MED、MEAM1、MEAM2 和 IO）属于入侵分支。正确鉴定这些隐种是实施植物生物安全和边境保护可靠措施的关键步骤；然而，目前还没有标准化的烟粉虱特异性引物，这导致了物种鉴定过程的不一致。
我们报道了三套聚合酶链反应（PCR）引物，用于扩增 mtCOI 区域，可用于鉴定 MED、MEAM1 和 IO 种，并用这些引物对 91 个 MED、35 个 MEAM1 和 5 个 IO 个体进行了测试。PCR 和扩增子测序共鉴定出 MED、MEAM1 和 IO 中的 21、6 和 1 个单倍型，其中 6 个单倍型是烟粉虱数据库中的新单倍型。
这些引物对通过 mtCOI 筛选目标入侵隐种，实现了标准化和稳健的分子物种鉴定，并将改进检疫决策。该诊断工具的使用可以扩展到该复合种中的其他种。 © 2017 化学工业协会。
