Kami Reddy Karthik Reddy, Piyarathna Danthasinghe Waduge Badrajee, Kamal Abu Hena Mostafa, Putluri Vasanta, Ravi Shiva Shankar, Bollag Roni J, Terris Martha K, Lotan Yair, Putluri Nagireddy
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Advanced Technology Cores, Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Metabolites. 2022 Jun 14;12(6):544. doi: 10.3390/metabo12060544.
Bladder Cancer (BLCA) is the ninth most frequently diagnosed cancer globally and the sixth most common cancer in the US. African Americans (AA) exhibit half the BLCA incidence compared to European Americans (EA), but they have a 70% higher risk of cancer-related death; unfortunately, this disparity in BLCA mortality remains poorly understood. In this study, we have used an ethnicity-balanced cohort for unbiased lipidomics profiling to study the changes in the lipid fingerprint for AA and EA BLCA tissues collected from similar geographical regions to determine a signature of ethnic-specific alterations. We identified 86 lipids significantly altered between self-reported AA and EA BLCA patients from Augusta University (AU) cohort. The majority of altered lipids belong to phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), ly sophosphatidylcholines (lysoPCs), phosphatidylserines (PSs), and diglycerides (DGs). Interestingly, levels of four lysoPCs (lyso PCs 20:3, lyso PCs 22:1, lyso PCs 22:2, and lyso PCs 26:1) were elevated while, in contrast, the majority of the PCs were reduced in AA BLCA. Significant alterations in long-chain monounsaturated (MonoUN) and polyunsaturated (PolyUN) lipids were also observed between AA and EA BLCA tumor tissues. These first-in-field results implicate ethnic-specific lipid alterations in BLCA.
膀胱癌（BLCA）是全球第九大最常被诊断出的癌症，也是美国第六大常见癌症。与欧洲裔美国人（EA）相比，非裔美国人（AA）的BLCA发病率只有其一半，但他们的癌症相关死亡风险却高出70%；不幸的是，BLCA死亡率方面的这种差异仍未得到充分理解。在本研究中，我们使用了一个种族均衡的队列进行无偏脂质组学分析，以研究从相似地理区域收集的AA和EA BLCA组织的脂质指纹变化，从而确定种族特异性改变的特征。我们从奥古斯塔大学（AU）队列中发现，自我报告的AA和EA BLCA患者之间有86种脂质发生了显著变化。大多数变化的脂质属于磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、溶血磷脂酰胆碱（lysoPC）、磷脂酰丝氨酸（PS）和甘油二酯（DG）。有趣的是，四种溶血磷脂酰胆碱（lysoPC 20:3、lysoPC 22:1、lysoPC 22:2和lysoPC 26:1）的水平升高，而相比之下，AA BLCA中的大多数磷脂酰胆碱水平降低。在AA和EA BLCA肿瘤组织之间还观察到长链单不饱和（MonoUN）和多不饱和（PolyUN）脂质有显著变化。这些该领域的首次研究结果表明BLCA存在种族特异性脂质改变。
