Wen Shi, Wang Zhenzhao, Feng Jianghua, Yang Yuanyuan, Lin Xianchao, Huang Heguang
Department of General Surgery, Fujian Medical University Union Hospital, No. 29, Xinquan Road, Gulou District, Fuzhou 351001, China.
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, No. 422, Siming South Road, Siming District, Xiamen 361005, China.
Metabolites. 2022 Jun 19;12(6):565. doi: 10.3390/metabo12060565.
Idiopathic thrombocytopenic purpura (ITP) is a common hematological disease and the abnormal platelet destruction in the spleen is a critical pathological mechanism for ITP. However, the metabolomic change in the spleen caused by ITP is still unclear. In the present study, the metabolomic information of 18 ITP and 20 normal spleen samples were detected by using H high-resolution magic angle spinning NMR spectroscopy (H MAS NMR). Compared with normal spleen, the concentrations of acetate, alanine, glutamine, glycerol, isoleucine, lysine, valine, phenylalanine, leucine, and methanol in ITP spleen tissue were elevated and 3-hydroxybutyric acid, ascorbate, asparagine, ethanol, glycogen, low-density lipoprotein, malonate, -inositol, glycerophosphocholine, pyroglutamate, and taurine were decreased. Amino acids metabolic pathways, such as branched-chain amino acids pathway, were identified as the main involved pathways based on enrichment analysis. The decrease in taurine level in the spleen was the most obvious metabolic signature involving ITP with high sensitivity and specificity to distinguish the spleen of ITP from the normal (CI: 0.825-0.982). Notably, the level of taurine in the spleen was negatively correlated with the efficacy of splenectomy ( = 0.622, = 0.006). Collectively, the data from our study revealed previously unknown ITP-related metabolomic changes in the spleen and found a potential diagnostic and efficacy-predictive biomarker for ITP treatment.
特发性血小板减少性紫癜（ITP）是一种常见的血液系统疾病，脾脏中血小板的异常破坏是ITP的关键病理机制。然而，ITP引起的脾脏代谢组学变化仍不清楚。在本研究中，采用高分辨率魔角旋转核磁共振光谱（H MAS NMR）检测了18例ITP脾脏样本和20例正常脾脏样本的代谢组学信息。与正常脾脏相比，ITP脾脏组织中乙酸盐、丙氨酸、谷氨酰胺、甘油、异亮氨酸、赖氨酸、缬氨酸、苯丙氨酸、亮氨酸和甲醇的浓度升高，而3-羟基丁酸、抗坏血酸盐、天冬酰胺、乙醇、糖原、低密度脂蛋白、丙二酸盐、肌醇、甘油磷酸胆碱、焦谷氨酸和牛磺酸的浓度降低。基于富集分析，氨基酸代谢途径如支链氨基酸途径被确定为主要涉及的途径。脾脏中牛磺酸水平的降低是ITP最明显的代谢特征，对区分ITP脾脏与正常脾脏具有高敏感性和特异性（CI：0.825 - 0.982）。值得注意的是，脾脏中牛磺酸水平与脾切除术的疗效呈负相关（r = 0.622，P = 0.006）。总体而言，我们研究的数据揭示了脾脏中以前未知的与ITP相关的代谢组学变化，并发现了一种用于ITP治疗的潜在诊断和疗效预测生物标志物。
