Lee Saeyoung, Chakma Nidhi, Joung Sunjeong, Lee Je Min, Lee Jundae
Department of Horticulture, Institute of Agricultural Science & Technology, Jeonbuk National University, Jeonju 54896, Korea.
Department of Horticultural Science, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Korea.
Plants (Basel). 2022 Jun 10;11(12):1551. doi: 10.3390/plants11121551.
Bacterial wilt caused by the β-proteobacterium is one of the most destructive soil-borne pathogens in peppers ( L.) worldwide. Cultivated pepper fields in Korea face a continuous spread of this pathogen due to global warming. The most efficient and sustainable strategy for controlling bacterial wilt is to develop resistant pepper varieties. Resistance, which is quantitatively inherited, occurs differentially depending on isolates. Therefore, in this study, we aimed to identify resistance quantitative trait loci (QTLs) in two F populations derived from self-pollination of a highly resistant pepper cultivar 'Konesian hot' using a moderately pathogenic 'HS' isolate and a highly pathogenic 'HWA' isolate of for inoculation, via genotyping-by-sequencing analysis. QTL analysis revealed five QTLs, , , , , and , conferring resistance to the 'HS' isolate with values of 13.05, 12.67, 15.07, 10.46, and 9.69%, respectively, and three QTLs, , , and , resistant to the 'HWA' isolate with phenotypic variances of 19.67, 16.50, and 12.56%, respectively. Additionally, six high-resolution melting (HRM) markers closely linked to the QTLs were developed. In all the markers, the mean disease index of the paternal genotype was significantly lower than that of the maternal genotype. The QTLs and HRM markers are expected to be useful for the development of pepper varieties with high resistance to bacterial wilt.
由β-变形菌引起的青枯病是全球辣椒（L.）中最具破坏性的土传病原菌之一。由于全球变暖，韩国的辣椒种植田面临着这种病原菌的持续传播。控制青枯病最有效和可持续的策略是培育抗病辣椒品种。抗性是数量遗传的，根据分离株的不同而有差异。因此，在本研究中，我们旨在通过测序基因分型分析，利用中等致病性的“HS”分离株和高致病性的“HWA”分离株对高度抗病辣椒品种“Konesian hot”自花授粉产生的两个F群体进行接种，以鉴定抗青枯病的数量性状位点（QTL）。QTL分析揭示了5个QTL，即、、、和，对“HS”分离株具有抗性，其贡献率分别为13.05%、12.67%、15.07%、10.46%和9.69%，以及3个QTL，即、和，对“HWA”分离株具有抗性，表型变异率分别为19.67%、16.50%和12.56%。此外，还开发了6个与这些QTL紧密连锁的高分辨率熔解（HRM）标记。在所有标记中，父本基因型的平均病情指数显著低于母本基因型。这些QTL和HRM标记有望用于培育对青枯病具有高抗性的辣椒品种。
