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成年斑马鱼肾脏中神经营养因子及其受体的表达分析

Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney.

作者信息

Cacialli Pietro, Lucini Carla

机构信息

Department of Pathology and Immunology, School of Medicine, University of Geneva, Rue Michel-Servet 1, 1206 Geneva, Switzerland.

Department of Veterinary Medicine, University of Naples Federico II, 80138 Naples, Italy.

出版信息

Vet Sci. 2022 Jun 15;9(6):296. doi: 10.3390/vetsci9060296.

DOI:10.3390/vetsci9060296
PMID:35737348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9227799/
Abstract

Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney.

摘要

神经营养因子及其受体参与神经元群体的发育和维持。不同的报告表明，所有神经营养因子/受体途径在脊椎动物的几种非神经元组织中也能发挥作用，包括肾脏。这些信号通路参与不同的事件以确保肾脏的正常功能，如生长、分化以及肾小管转运的调节。之前在一些鱼类中的研究已经鉴定出了肾脏中的神经营养因子和受体。在本研究中，我们首次比较了成年斑马鱼肾脏中所有神经营养因子/受体途径的表达谱（mRNA和蛋白质）。我们使用qPCR定量mRNA水平，并通过原位杂交确定每个神经营养因子/受体途径的表达模式。接下来，我们使用蛋白质印迹法和免疫组织化学检测蛋白质。我们的结果表明，在所分析的所有神经营养因子中，NT-3/TrkC在肾小球、肾小管以及造血细胞中表达最为丰富，这与在哺乳动物肾脏中的报道相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ae3a504f3d34/vetsci-09-00296-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ba4be4fd49fd/vetsci-09-00296-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ecf7367423ad/vetsci-09-00296-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/591a3f41cd9d/vetsci-09-00296-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/30996ce1a37e/vetsci-09-00296-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/7863d5d6080a/vetsci-09-00296-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/309e790b4baf/vetsci-09-00296-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/bc1f8f042539/vetsci-09-00296-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ae3a504f3d34/vetsci-09-00296-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ba4be4fd49fd/vetsci-09-00296-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ecf7367423ad/vetsci-09-00296-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/591a3f41cd9d/vetsci-09-00296-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/30996ce1a37e/vetsci-09-00296-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/7863d5d6080a/vetsci-09-00296-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/309e790b4baf/vetsci-09-00296-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/bc1f8f042539/vetsci-09-00296-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ba9/9227799/ae3a504f3d34/vetsci-09-00296-g008.jpg

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