Université de Carthage, Institut National des Sciences Appliquées et de Technologie, USCR Bactériologie Moléculaire & génomique, 1080 Tunis Cedex, Tunisia.
IFAPA Las Torres- Andalusian Institute of Agricultural and Fisheries Research and Training, Junta de Andalucía, Cra. Sevilla-Cazalla, km 12.2., 41200, Alcalá del Río, Seville, Spain.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005441.
A new actinobacterium strain, designated BMG 823, was isolated from a limestone sample collected in Tunisia. Its taxonomic position was scrutinized using a polyphasic approach. Colonies of strain BMG 823 were pink orange-coloured, regular and had a moist surface. Cells are Gram-stain-positive, catalase-negative and oxidase-negative. The strain grew at pH 5.5-9, 10-40 °C and in presence of up to 4 % NaCl (w/v). Chemotaxonomically, strain BMG 823 was characterized by cell-wall type III containing -diaminopimelic acid as diamino acid, glucose, ribose and rhamnose as whole-cell sugars, MK-9(H) as predominant menaquinone, and phosphatidylcholine, diphosphadidylglycerol, phosphatidethanolamine, phosphatidylcholine, phosphatidylinositol, unidentified glycolipid, unidentified aminophospholipids and unidentified glycophospholipid as major polar lipids. The fatty acid profile consisted of iso-C and iso-C 9. Phylogenetic trees based on 16S rRNA gene and genome sequences placed strain BMG 823 within the genus and separated it from all type strains of validly published species. Comparison of 16S rRNA gene sequence similarity, digital DNA-DNA hybridization and average nucleotide identity indicated that strain BMG 823 was most closely related to DSM 106127 and BMG 822 with pairwise values well below the species differentiation thresholds. The distinct phenotypic and genotypic features of strain BMG 823 (=DSM 46838=CECT 8881) within the genus warrant its recognition as the type strain for the new species for which we propose the name sp. nov.
一株新的放线菌菌株，命名为 BMG 823，从突尼斯采集的石灰岩样本中分离得到。采用多相分类法研究其分类地位。菌株 BMG 823 的菌落呈粉红色橙色，规则，表面湿润。细胞革兰氏阳性，过氧化氢酶阴性，氧化酶阴性。该菌株在 pH5.5-9、10-40°C 和高达 4%（w/v）NaCl 的条件下生长。化学分类学上，菌株 BMG 823 的细胞壁类型为 III，含有 -二氨基庚二酸作为二氨基酸，全细胞糖为葡萄糖、核糖和鼠李糖，主要的膜烯醌为 MK-9(H)，主要的极性脂为磷脂酰胆碱、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇、未鉴定的糖脂、未鉴定的氨基磷脂和未鉴定的糖基磷脂。脂肪酸图谱由 iso-C 和 iso-C9 组成。基于 16S rRNA 基因和基因组序列构建的系统发育树将菌株 BMG 823 归入 属，并将其与所有有效发表的种的模式菌株区分开来。16S rRNA 基因序列相似性、数字 DNA-DNA 杂交和平均核苷酸同一性的比较表明，菌株 BMG 823 与 DSM 106127 和 BMG 822 最为密切相关，两者的成对值均远低于种间分化阈值。菌株 BMG 823（=DSM 46838=CECT 8881）在 属内具有独特的表型和基因型特征，证明其为新种的模式菌株，我们建议将其命名为 sp. nov.。
