Xu Ce, Wang Ying, Zhang Ruirui, Zhang Jiquan, Sun Yuying
School of Life Sciences, Institute of Life Sciences and Green Development, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Hebei University, Baoding 071002, China.
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Hebei University, Baoding 071002, China.
Fish Shellfish Immunol Rep. 2023 Jan 4;4:100081. doi: 10.1016/j.fsirep.2023.100081. eCollection 2023 Dec.
Peroxiredoxins (Prxs) widely exist in organisms and can prevent oxidative damage. Here, the characterization and biological function of NdPrx3 from were analyzed. The coding sequence of consists of 684 bp open reading frame (ORF), encoding 227 amino acids with a predicted molecular weight of 24.7 kDa and theoretical pI 6.49. Multiple sequence alignments showed that the conserved domains of NdPrx3, including catalytic triad, dimer interface, decamer interface, peroxidatic, and resolving cysteines, were similar to those of other organisms. The phylogenetic relationship demonstrated that NdPrx3 clustered in the Prx3 class. The highest relative expression of mRNA was confirmed in gill among the nine tissues from healthy shrimp. The transcript level of was significantly upregulated from 0 h to 48 h and decreased in 72 h under copper challenge, indicating that may play an important role in the copper challenge of . In addition, NdPrx3 was recombinantly expressed in and purified to one band on SDS-PAGE. The DNA protection of rNdPrx3 was verified. The enzymatic assay of the recombinant NdPrx3 indicated that it had the oxidoreductase function and was stable at a low temperature (10-30 °C).
过氧化物酶（Prxs）广泛存在于生物体中，能够预防氧化损伤。在此，对来自[具体来源未给出]的NdPrx3的特性和生物学功能进行了分析。[具体来源未给出]的编码序列由684个碱基对的开放阅读框（ORF）组成，编码227个氨基酸，预测分子量为24.7 kDa，理论pI为6.49。多序列比对显示，NdPrx3的保守结构域，包括催化三联体、二聚体界面、十聚体界面、过氧化物酶和还原半胱氨酸，与其他生物体的相似。系统发育关系表明，NdPrx3聚类在Prx3类中。在健康虾的九个组织中，鳃中[具体来源未给出]mRNA的相对表达最高。在铜胁迫下，[具体来源未给出]的转录水平在0小时到48小时显著上调，在72小时下降，表明[具体来源未给出]可能在[具体来源未给出]的铜胁迫中发挥重要作用。此外，NdPrx3在[具体宿主未给出]中进行重组表达，并在SDS-PAGE上纯化至单一条带。验证了重组NdPrx3的DNA保护作用。重组NdPrx3的酶活性测定表明它具有氧化还原酶功能，并且在低温（10 - 30°C）下稳定。
