Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Protein and Proteomics Research Center for Commercial and Industrial Purposes, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sci Rep. 2022 Jun 23;12(1):10701. doi: 10.1038/s41598-022-14550-x.
Burkholderia pseudomallei (B. pseudomallei) is a Gram-negative pathogen that causes melioidosis, a deadly but neglected tropical disease. B. pseudomallei is intrinsically resistant to a growing list of antibiotics, and alternative antimicrobial agents are being sought with urgency. In this study, we synthesize andrographolide-stabilized silver nanoparticles (andro-AgNPs, spherically shaped with 16 nm average diameter) that show excellent antimicrobial activity against B. pseudomallei, including ceftazidime-resistant strains, being 1-3 orders of magnitude more effective than ceftazidime and 1-2 orders of magnitude more effective than other green-synthesized AgNPs. The andro-AgNPs are meanwhile non-toxic to mammalian cell lines. The mode of action of Andro-AgNPs toward B. pseudomallei is unraveled by killing kinetics, membrane neutralization, silver ions (Ag) release, reactive oxygen species (ROS) induction, membrane integrity, and cell morphology change studies. The antimicrobial activity and mode of action of andro-AgNPs against B. pseudomallei reported here may pave the way to alternative treatments for melioidosis.
类鼻疽伯克霍尔德菌（B. pseudomallei）是一种革兰氏阴性病原体，可引起类鼻疽病，这是一种致命但被忽视的热带病。B. pseudomallei 对越来越多的抗生素具有固有耐药性，因此正在紧急寻找替代的抗菌药物。在本研究中，我们合成了穿心莲内酯稳定的银纳米粒子（andro-AgNPs，呈球形，平均直径为 16nm），其对 B. pseudomallei 具有出色的抗菌活性，包括对头孢他啶耐药的菌株，其抗菌效果比头孢他啶高 1-3 个数量级，比其他绿色合成的 AgNPs 高 1-2 个数量级。与此同时，andro-AgNPs 对哺乳动物细胞系没有毒性。通过杀菌动力学、膜中和、银离子（Ag）释放、活性氧（ROS）诱导、膜完整性和细胞形态变化研究，揭示了 Andro-AgNPs 对 B. pseudomallei 的作用模式。本文报道的 andro-AgNPs 对 B. pseudomallei 的抗菌活性和作用模式可能为治疗类鼻疽病开辟了新的治疗途径。
