LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal; ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
J Hazard Mater. 2022 Aug 15;436:129185. doi: 10.1016/j.jhazmat.2022.129185. Epub 2022 May 19.
Musk fragrances have been detected in drinking water (DW) at trace concentrations. However, their impact on the microbial quality of DW has been disregarded. This work provides a pioneer evaluation of the effects of two synthetic musks contaminants, tonalide (AHTN) and galaxolide (HHCB), in microbial biofilms formed on two different surfaces, polyvinyl chloride (PVC) and stainless steel AISI 316 (SS316). Three bacterial species isolated from DW (Acinetobacter calcoaceticus, Burkholderia cepacia and Stenotrophomonas maltophilia), were used to develop 7-day-old single and mixed species biofilms. The impact of musks was assessed directly on biofilms but also on the bacteria motility, biofilm formation ability and biofilm susceptibility to chlorination. AHTN musk caused the most remarkable effects by increasing the cellular density and viability of mixed biofilms, and the extracellular polysaccharides content of biofilms on SS316. Most of the alterations caused by the direct exposure of biofilms to musks were observed when SS316 was used as an adhesion surface. In contrast, the ability to form biofilms and their susceptibility to chlorine were more affected for bacteria from HHCB-exposed biofilms on PVC. The overall results demonstrate that the presence of musks at residual concentrations influences DW bacterial dynamics, with the potential to impact the DW quality and safety. The type of plumbing material may further impact the effects of musks.
麝香香精已在饮用水 (DW) 中痕量检测到。然而，它们对 DW 微生物质量的影响尚未得到重视。本工作首次评估了两种合成麝香污染物—— tonalide (AHTN) 和 galaxolide (HHCB) 对两种不同表面（聚氯乙烯 (PVC) 和不锈钢 AISI 316 (SS316)）上形成的微生物生物膜的影响。从 DW 中分离出的三种细菌（醋酸钙不动杆菌、洋葱伯克霍尔德菌和嗜麦芽寡养单胞菌）用于开发 7 天龄的单种和混合种生物膜。直接在生物膜上评估麝香的影响，还评估了麝香对细菌运动性、生物膜形成能力和生物膜对氯化的敏感性的影响。AHTN 麝香通过增加混合生物膜的细胞密度和活力以及 SS316 生物膜的胞外多糖含量，引起了最显著的影响。当 SS316 用作附着表面时，观察到生物膜直接暴露于麝香时引起的大多数变化。相比之下，暴露于 HHCB 的 PVC 生物膜上的细菌形成生物膜的能力及其对氯的敏感性受到的影响更大。总体结果表明，残余浓度麝香的存在会影响 DW 中的细菌动态，有可能影响 DW 的质量和安全性。管道材料的类型可能会进一步影响麝香的影响。
