Department of Plant Physiology and Biophysics, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 Street, 20-033 Lublin, Poland.
Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 Street, 20-033 Lublin, Poland.
J Hazard Mater. 2022 Aug 15;436:129232. doi: 10.1016/j.jhazmat.2022.129232. Epub 2022 May 26.
Copper stress in the presence of exogenous methyl jasmonate and Serratia plymuthica in a complete trifactorial design with copper (0, 50 µM), methyl jasmonate (0, 1, 10 µM) and Serratia plymuthica (without and with inoculation) was studied on the physiological parameters of Phaseolus coccineus. Copper application reduced biomass and allantoin content, but increased chlorophyll and carotenoids contents as well as catalase and peroxidases activities. Jasmonate did not modify biomass and organic acids levels under copper treatment, but additional inoculation elevated biomass and content of tartrate, malate and succinate. Jasmonate used alone or in combination with bacteria increased superoxide dismutase activity in copper application. With copper, allantoin content elevated at lower jasmonate concentration, but with additional inoculation - at higher jasmonate concentration. Under copper stress, inoculation resulted in higher accumulation of tartrate, malate and citrate contents in roots, which corresponded with lower allantoin concentration in roots. Combined with copper, inoculation reduced catalase and guaiacol peroxidase activities, whereas organic acids content was higher. Under metal stress, with bacteria, jasmonate reduced phenolics content, elevated superoxide dismutase and guaiacol peroxidase activities. The data indicate that jasmonate and S. plymuthica affected most physiological parameters of P. coccineus grown with copper and revealed some effect on biomass.
在完全三因子设计中，外源茉莉酸甲酯和粘质沙雷氏菌存在的情况下，铜胁迫对菜豆的生理参数进行了研究。铜的应用降低了生物量和尿囊素含量，但增加了叶绿素和类胡萝卜素含量以及过氧化氢酶和过氧化物酶的活性。茉莉酸甲酯在铜处理下没有改变生物量和有机酸水平，但额外的接种提高了生物量和酒石酸盐、苹果酸盐和琥珀酸盐的含量。单独使用茉莉酸甲酯或与细菌一起使用会增加铜应用中的超氧化物歧化酶活性。在铜存在的情况下，较低浓度的茉莉酸甲酯会增加尿囊素的含量，但在额外接种的情况下，较高浓度的茉莉酸甲酯会增加尿囊素的含量。在铜胁迫下，接种导致根中酒石酸、苹果酸和柠檬酸含量升高，这与根中尿囊素浓度降低相对应。与铜结合时，接种降低了过氧化氢酶和愈创木酚过氧化物酶的活性，而有机酸的含量更高。在金属胁迫下，细菌中的茉莉酸甲酯降低了酚类物质的含量，提高了超氧化物歧化酶和愈创木酚过氧化物酶的活性。这些数据表明，茉莉酸甲酯和粘质沙雷氏菌影响了铜处理下菜豆的大多数生理参数，并对生物量产生了一定的影响。
