Gao Yuan, Zhang Zhonghuai, Wang Chunren, Zhao Kai
Institute of Nanobiomaterials and Immunology, School of Life Science, Taizhou University, Taizhou 318000, China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Animals (Basel). 2022 Jun 17;12(12):1571. doi: 10.3390/ani12121571.
() is one of the species in , subfamily Cyathostominae, but its taxonomic status in is controversial. Mitochondrial (mt) genome is an excellent gene marker which could be used to address the taxonomy controversy. In the present study, the complete mt genome of was determined, and sequence and phylogenetic analyses were performed based on mtDNA data to determine the classification of . The circular complete mt genome of was 13875 bp in size, containing 12 protein-coding genes (12 PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions (NCRs). The A + T content of complete mt genome was 76.64%. There were 19 intergenic spacers with lengths of 2-53 bp and 2 overlaps with lengths of 1-2 bp in the impact complete mt genome. ATT and TAA were the most common start and termination codons of 12 PCGs, respectively. Comparative analyses of mt genomes nucleotide sequence and amino acid sequence showed that there were higher identities between and five other , rather than with . Phylogenetic analyses based on concatenated nucleotide sequences of 12 PCGs of 23 species in the family Strongylidae showed that was closely related to species, rather than . We concluded that was a member in based on comparative and phylogenetic analyses of mt genome sequences. The data of the complete mt genome sequence of provide a new and useful genetic marker for further research on Cyathostominae nematodes.
（某物种名称）是杯环线虫亚科中（某属名称）的物种之一，但其在（某属名称）中的分类地位存在争议。线粒体（mt）基因组是一种出色的基因标记，可用于解决分类学争议。在本研究中，测定了（某物种名称）的完整线粒体基因组，并基于线粒体DNA数据进行了序列和系统发育分析，以确定（某物种名称）的分类。（某物种名称）的环状完整线粒体基因组大小为13875 bp，包含12个蛋白质编码基因（12个PCG）、22个转移RNA（tRNA）基因、2个核糖体RNA（rRNA）基因和2个非编码区（NCR）。（某物种名称）完整线粒体基因组的A + T含量为76.64%。在（某物种名称）完整线粒体基因组中，有19个长度为2 - 53 bp的基因间隔区和2个长度为1 - 2 bp的重叠区。ATT和TAA分别是12个PCG最常见的起始和终止密码子。线粒体基因组核苷酸序列和氨基酸序列的比较分析表明，（某物种名称）与其他五个（某属物种名称）之间的同源性较高，而与（另一属物种名称）的同源性较低。基于圆线科23个物种的12个PCG串联核苷酸序列进行的系统发育分析表明，（某物种名称）与（某属物种名称）关系密切，而与（另一属物种名称）关系较远。基于线粒体基因组序列的比较和系统发育分析，我们得出结论，（某物种名称）是（某属名称）的成员。（某物种名称）完整线粒体基因组序列的数据为进一步研究杯环线虫亚科线虫提供了一个新的有用的遗传标记。
