Li Xuankai, Wang Lidan, Chen Yijun, Wang Xianxiang, Zhou Xuan, Xie Yue
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Science, Sichuan Agricultural University, Ya'an, China.
Mitochondrial DNA B Resour. 2022 Feb 24;7(2):419-421. doi: 10.1080/23802359.2022.2044928. eCollection 2022.
spp. (Nematoda: Strongylida: Cyathostominae) are the common and important parasitic nematodes found in horses and donkeys worldwide. In this study, the complete mitochondrial genome of Looss 1900, a representative member of this genus from the donkey in Southwest China was determined using the next-generation DNA sequencing technology. The genome was 13,851 bp in size and consisted of 36 genes including 12 protein-coding genes (6, 1-3, , 1-6 and 4L), 22 transfer RNA genes and two ribosomal RNA genes (L and S), as well as two non-coding regions. Phylogenetic analysis showed that and Boulenger 1917 were closely related, and then both grouped with other congeneric species and formed a monophyletic relationship with either species of , , , or , demonstrating their phylogenetic stability within Cyathostominae. These cumulative mitochondrial DNA data provide novel and useful genetic markers for molecular diagnostic, systematic and evolutionary biological studies of Cyathostominae nematodes.
spp.（线虫纲：圆线目：杯环线虫亚科）是在世界各地的马和驴体内发现的常见且重要的寄生线虫。在本研究中，利用下一代DNA测序技术测定了来自中国西南部驴的该属代表性成员1900年洛斯线虫的完整线粒体基因组。该基因组大小为13,851 bp，由36个基因组成，包括12个蛋白质编码基因（6、1 - 3、、1 - 6和4L）、22个转运RNA基因和两个核糖体RNA基因（L和S），以及两个非编码区。系统发育分析表明，1917年的布伦杰线虫与之密切相关，然后两者与其他同属物种归为一类，并与、、、或的任何一个物种形成单系关系，表明它们在杯环线虫亚科内的系统发育稳定性。这些累积的线粒体DNA数据为杯环线虫亚科线虫的分子诊断、系统发育和进化生物学研究提供了新颖且有用的遗传标记。
