Yang Weiguang, Guo Guangzhen, Chen Jiayi, Wang Shengnan, Gao Zhenhua, Zhao Zhihui, Yin Fuquan
College of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, China.
The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
Animals (Basel). 2022 Jun 20;12(12):1591. doi: 10.3390/ani12121591.
The purpose of this study was to evaluate the effects of fucoidan supplementation on serum biochemical parameters, small intestinal barrier function, and cecal microbiota of weaned goat kids. A total of 60 2-month-old weaned castrated male goat kids (Chuanzhong black goat) were used in this 30-day experiment. The goat kids were randomly divided into four groups: a control group (CON) fed the basal diet, and three other groups supplemented with 0.1%, 0.3%, and 0.5% fucoidan in the basal diet (denoted as F1, F2, and F3 groups, respectively). The results indicated that dietary fucoidan supplementation decreased (p < 0.05) the activity of lactate dehydrogenase (LDH) and the content of glucose (GLU) as measured on day 15. As measured on day 30, dietary fucoidan increased (p < 0.05) the content of total protein (TP) and decreased the activity of aspartate aminotransferase (AST), and supplementation with 0.3% and 0.5% fucoidan decreased (p < 0.05) the activity of LDH. Dietary fucoidan decreased (p < 0.05) the content of D-lactic acid (D-LA) and the activity of diamine oxidase (DAO). Dietary fucoidan increased (p < 0.05) the activity of catalase (CAT) in the duodenum. Dietary 0.3% and 0.5% fucoidan enhanced (p < 0.05) the activity of glutathione peroxidase (GSH-Px) in the ileum, the activity of total superoxide dismutase (T-SOD) in the jejunum and ileum, and the activity of CAT in the ileum. Dietary 0.3% and 0.5% fucoidan reduced the contents of malondialdehyde (MDA) in the duodenum, jejunum, and ileum and the content of hydrogen peroxide (H2O2) in the duodenum. Dietary fucoidan increased (p < 0.05) the content of secretory immunoglobulin A (sIgA) in the duodenum. Supplementation of 0.3% and 0.5% fucoidan upregulated (p < 0.05) the gene expression of ZO-1 and claudin-1 in the duodenum, jejunum, and ileum, and dietary supplementation of 0.3% and 0.5% fucoidan upregulated (p < 0.05) the gene expression of occludin in the jejunum and ileum. The 16S rRNA high-throughput sequencing results showed that at the phylum level, dietary fucoidan increased (p < 0.05) the abundance of Bacteroidetes while decreasing (p < 0.05) the abundance of Firmicutes. At the genus level, dietary 0.3% and 0.5% fucoidan increased (p < 0.05) the abundances of Unspecified_Ruminococcaceae, Unspecified_Bacteroidale, Unspecified_Clostridiales, and Akkermansia. In conclusion, dietary fucoidan supplementation had positive effects on intestinal permeability, antioxidant capacity, immunity function, tight junctions, and the cecal microflora balance in weaned goat kids.
本研究旨在评估补充岩藻多糖对断奶山羊羔血清生化参数、小肠屏障功能和盲肠微生物群的影响。在这项为期30天的实验中，共使用了60只2月龄断奶去势雄性山羊羔（川中黑山羊）。山羊羔被随机分为四组：对照组（CON）饲喂基础日粮，其他三组在基础日粮中分别补充0.1%、0.3%和0.5%的岩藻多糖（分别记为F1、F2和F3组）。结果表明，在第15天测定时，日粮中补充岩藻多糖降低了（p<0.05）乳酸脱氢酶（LDH）的活性和葡萄糖（GLU）的含量。在第30天测定时，日粮中补充岩藻多糖增加了（p<0.05）总蛋白（TP）的含量并降低了天冬氨酸转氨酶（AST）的活性，补充0.3%和0.5%的岩藻多糖降低了（p<0.05）LDH的活性。日粮中补充岩藻多糖降低了（p<0.05）D-乳酸（D-LA）的含量和二胺氧化酶（DAO）的活性。日粮中补充岩藻多糖增加了（p<0.05）十二指肠中过氧化氢酶（CAT）的活性。日粮中补充0.3%和0.5%的岩藻多糖增强了（p<0.05）回肠中谷胱甘肽过氧化物酶（GSH-Px）的活性、空肠和回肠中总超氧化物歧化酶（T-SOD）的活性以及回肠中CAT的活性。日粮中补充0.3%和0.5%的岩藻多糖降低了十二指肠、空肠和回肠中丙二醛（MDA）的含量以及十二指肠中过氧化氢（H2O2）的含量。日粮中补充岩藻多糖增加了（p<0.05）十二指肠中分泌型免疫球蛋白A（sIgA）的含量。补充0.3%和0.5%的岩藻多糖上调了（p<0.05）十二指肠、空肠和回肠中紧密连接蛋白1（ZO-1）和闭合蛋白1（claudin-1）的基因表达，日粮中补充0.3%和0.5%的岩藻多糖上调了（p<0.05）空肠和回肠中闭锁蛋白（occludin）的基因表达。16S rRNA高通量测序结果表明，在门水平上，日粮中补充岩藻多糖增加了（p<0.05）拟杆菌门的丰度，同时降低了（p<0.05）厚壁菌门的丰度。在属水平上，日粮中补充0.3%和0.5%的岩藻多糖增加了（p<0.05）未分类的瘤胃球菌科、未分类的拟杆菌目、未分类的梭菌目和阿克曼氏菌属的丰度。总之，日粮中补充岩藻多糖对断奶山羊羔的肠道通透性、抗氧化能力、免疫功能、紧密连接以及盲肠微生物群平衡具有积极影响。
