College of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural University, Nanjing, 210095, China.
Biol Trace Elem Res. 2022 Apr;200(4):1826-1834. doi: 10.1007/s12011-021-02771-6. Epub 2021 Jun 8.
This experiment was to study the effects of zinc oxide nanoparticles (ZnO-NPs) on growth, intestinal barrier, oxidative status, and mineral deposition. In total, 256 one-day-old chicks were randomly allotted to 4 dietary groups and fed with basal diet plus 80 mg/kg ZnSO (ZnSO group) or plus 40, 80, and 160 mg/kg ZnO-NPs, respectively, for 21 days. Compared with the ZnSO group, dietary 40, 80, and 160 mg/kg ZnO-NPs did not alter growth (average daily gain, body weight, and gain to feed ratio), and serum activities of glutamic-pyruvic transaminase, alkaline phosphatase and glutamic oxalacetic transaminase (P > 0.05). However, dietary 80 and 160 mg/kg ZnO-NPs linearly decreased serum D-lactate content and diamine oxidase activity (P < 0.01). Moreover, 80 mg/kg ZnO-NPs enhanced zonula occludens-1 (ZO-1) mRNA expression in jejunal mucosa (P = 0.02). Dietary ZnO-NPs increased total antioxidant capacity activity (P = 0.01), and 80 mg/kg ZnO-NPs decreased malondialdehyde content in jejunal mucosa as compared to the ZnSO group (P = 0.02). In contrast, dietary ZnO-NPs did not alter mRNA expressions of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, heme oxygennase-1 (HO-1) and NAD (P)H: quinone oxidoreductase 1 (NQO1) (P > 0.05). No significant difference was found in selected mineral concentrations (Mn, Cu, Fe and Zn) in the liver among ZnSO and 3 ZnO-NP groups (P > 0.05). However, 160 mg/kg ZnO-NPs increased fecal contents of Zn, Fe and Cu (P < 0.01), but did not affect fecal Mn level (P > 0.05). Therefore, results suggested that ZnO-NPs could be an additive to enhance the intestinal barrier and antioxidant capacity of broiler chicks, whereas the inclusion of 80 mg/kg would be more efficient.
本试验旨在研究氧化锌纳米粒子（ZnO-NPs）对生长、肠道屏障、氧化状态和矿物质沉积的影响。将 256 只 1 日龄雏鸡随机分为 4 个日粮组，分别饲喂基础日粮+80mg/kg ZnSO（ZnSO 组）或 40、80 和 160mg/kg ZnO-NPs，试验期 21d。与 ZnSO 组相比，饲粮添加 40、80 和 160mg/kg ZnO-NPs 对平均日增重、体重和料重比均无显著影响（P＞0.05），但血清谷丙转氨酶、碱性磷酸酶和谷草转氨酶活性均线性降低（P＜0.01）。此外，80 和 160mg/kg ZnO-NPs 线性增加了空肠黏膜中二胺氧化酶活性和紧密连接蛋白-1（ZO-1）mRNA 表达（P＜0.01）。饲粮 ZnO-NPs 增加了总抗氧化能力活性（P=0.01），80mg/kg ZnO-NPs 降低了空肠黏膜丙二醛含量（P=0.02）。相反，饲粮 ZnO-NPs 对超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶、血红素加氧酶-1（HO-1）和 NAD（P）H：醌氧化还原酶 1（NQO1）mRNA 表达没有影响（P＞0.05）。ZnSO 和 3 个 ZnO-NP 组间肝脏中 Mn、Cu、Fe 和 Zn 浓度没有显著差异（P＞0.05）。然而，160mg/kg ZnO-NPs 增加了粪便中 Zn、Fe 和 Cu 的含量（P＜0.01），但不影响粪便 Mn 水平（P＞0.05）。综上所述，ZnO-NPs 可作为添加剂增强肉鸡的肠道屏障和抗氧化能力，添加 80mg/kg 更为有效。
