Paiva Lisete, Lima Elisabete, Motta Madalena, Marcone Massimo, Baptista José
Gorreana Tea Plantation, Gorreana, 9625-304 Maia, Portugal.
Department of Physics, Chemistry and Engineering (DCFQE), Faculty of Science and Technology, University of Azores, 9500-321 Ponta Delgada, São Miguel, Azores, Portugal.
Antioxidants (Basel). 2022 May 27;11(6):1066. doi: 10.3390/antiox11061066.
The molecular constituents of , in particular epigallocatechin-3--gallate (EGCG) and, more remarkably, the galloylated theaflavins, mainly theaflavin-3,3'-di--gallate (TF-3,3'-DG), have been reported to inhibit SARS-CoV-2 3-chymotrypsin-like protease (3CL), an enzyme required for the cleavage of its polyproteins, to produce vital individual functional proteins for viral cell replication. Our results for total catechin content revealed the values of 174.72, 200.90, and 211.75 mg/g dry weight (DW) in spring, and the values of 183.59, 191.36, and 215.09 mg/g DW in summer, for tea plantation zones 1, 2, and 3, respectively. For the TF-3,3'-DG content, the values of 2.68, 1.13, and 3.72 mg/g DW were observed in spring, and the values of 3.78, 2.06, and 8.91 mg/g DW in summer for zones 1, 2, and 3, respectively. In the same zone, different contents of TF-3,3'-DG were observed across plucking months of April, June, and August, with values of 1.13, 2.77, and 4.18 mg/g DW, respectively, showing higher values in summer. Different values of TF-3,3'-DG contents were also observed in the same tea plantation zone but from different plant parts, revealing higher values in the bud and the first and second leaves (3.62 mg/g DW) and lower values in the third and fourth leaves (1.14 mg/g DW). The TF-3,3'-DG content increased from 3.31 to 4.98 mg/g DW with increased fermentation time from 1 to 3 h, respectively, and increased for lower temperature and longer fermentation time. The aim of this study was to investigate the processing conditions that lead to maximum TF-3,3'-DG content and, given its potential impact as an inhibitor of the 3CL enzyme, to create a novel antiviral Azorean black tea.
据报道，其分子成分，特别是表没食子儿茶素-3-没食子酸酯（EGCG），更显著的是没食子酰化茶黄素，主要是茶黄素-3,3'-二没食子酸酯（TF-3,3'-DG），能够抑制严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的3-胰凝乳蛋白酶样蛋白酶（3CL），该酶是切割其多聚蛋白以产生病毒细胞复制所需的重要单个功能蛋白所必需的。我们测定的总儿茶素含量结果显示，茶园1区、2区和3区春季的含量分别为174.72、200.90和211.75毫克/克干重（DW），夏季的含量分别为183.59、191.36和215.09毫克/克DW。对于TF-3,3'-DG含量，1区、2区和3区春季分别观测到2.68、1.13和3.72毫克/克DW，夏季分别为3.78、2.06和8.91毫克/克DW。在同一区域，4月、6月和8月采摘月份的TF-3,3'-DG含量不同，分别为1.13、2.77和4.18毫克/克DW，夏季含量更高。在同一茶园区域但不同植株部位也观测到不同的TF-3,3'-DG含量值，芽以及第一和第二片叶子中的含量较高（3.62毫克/克DW），第三和第四片叶子中的含量较低（1.14毫克/克DW）。随着发酵时间从1小时增加到3小时，TF-3,3'-DG含量分别从3.31毫克/克DW增加到4.98毫克/克DW，并且在较低温度和较长发酵时间下含量增加。本研究的目的是探究能使TF-3,3'-DG含量达到最高的加工条件，并鉴于其作为3CL酶抑制剂的潜在影响，创制一种新型抗病毒亚速尔红茶。
