Zhan Ge, Long Men, Shan Kai, Xie Chong, Yang Runqiang
School of Biological Science and Food Engineering, Chuzhou University, Chuzhou 239000, China.
Whole Grain Food Engineering Research Center, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Antioxidants (Basel). 2022 May 27;11(6):1068. doi: 10.3390/antiox11061068.
Chrysanthemum has a long history of being used to attenuate various oxidative stress-related discomforts and diseases; however, its mechanisms remain unclear. In this study, the antioxidant effect of chrysanthemum aqueous extract was investigated, and the potential mechanisms were explored via a metabolomics study. Chrysanthemum extract could significantly inhibit hydrogen peroxide (HO)-mediated cell death in L-O2 hepatocytes. Propidium iodide staining and annexin V-PI dual staining revealed that the antioxidant effect of chrysanthemum extract was related to the relief of cell cycle arrest and inhibition of non-apoptotic cell damage. The activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were also upregulated by chrysanthemum extract. Through metabolomics studies, it was found that chrysanthemum extract mainly targeted the arginine synthesis pathway and purine metabolism pathway, in which antioxidation-related endogenous substrates including L-arginosuccinate, citrulline and inositol monophosphate were significantly upregulated by chrysanthemum extract. These results indicated that chrysanthemum extract can antagonize oxidative stress through multiple pathways and have potential therapeutic applications.
菊花用于减轻各种与氧化应激相关的不适和疾病已有很长的历史；然而，其作用机制仍不清楚。在本研究中，对菊花水提取物的抗氧化作用进行了研究，并通过代谢组学研究探索了其潜在机制。菊花提取物能显著抑制过氧化氢（H₂O₂）介导的L-O₂肝细胞死亡。碘化丙啶染色和膜联蛋白V-碘化丙啶双重染色显示，菊花提取物的抗氧化作用与缓解细胞周期阻滞和抑制非凋亡性细胞损伤有关。菊花提取物还上调了包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）在内的抗氧化酶的活性。通过代谢组学研究发现，菊花提取物主要作用于精氨酸合成途径和嘌呤代谢途径，其中菊花提取物显著上调了包括L-精氨琥珀酸、瓜氨酸和肌醇单磷酸在内的与抗氧化相关的内源性底物。这些结果表明，菊花提取物可通过多种途径拮抗氧化应激并具有潜在治疗应用价值。
