Hussein Hebat-Allah A, Alshammari Shifaa O, Elkady Fatma M, Ramadan Amany A, Kenawy Sahar K M, Abdelkawy Aisha M
Botany and Microbiology Department, Faculty of Science (Girls Branch), Al-Azhar University, Cairo 11754, Egypt.
Biology Department, University College of Nairiyah, University of Hafr Al-Batin, Nairiyah 31991, Saudi Arabia.
Antioxidants (Basel). 2022 Jun 10;11(6):1144. doi: 10.3390/antiox11061144.
Ionizing radiation is abiotic stress limiting the growth and productivity of crop plants. Stigmasterol has positive effects on the plant growth of many crops. The role of stigmasterol in alleviating the effects of ionizing radiation on plant metabolism and development is still unclear. Therefore, the study aimed to investigate the effects of pretreatments with γ-radiation (0, 25, and 50 Gy), foliar application of stigmasterol (0, 100, and 200 ppm), and their interaction on the growth, and biochemical constituents of wheat ( L., var. Sids 12) plants. Gamma radiation at 25 Gy showed no significant difference in plant height, root length, no. of leaves, shoot fresh weight, root fresh weight, Chl , ABA, soluble phenols, and MDA compared to the control values. Gamma rays at 50 Gy inhibited shoot and root lengths, flag leaf area, shoot fresh and dry weights, photosynthetic pigments, total soluble sugars, proline, and peroxidase activity. However, it stimulated total phenols, catalase activity, and lipid peroxidation. On the other hand, stigmasterol at 100 ppm showed no significant effects on some of the physiological attributes compared to control plants. Stigmasterol at 200 ppm improved plant growth parameters, photosynthetic pigments, proline, phenols, antioxidant enzyme, gibberellic acid, and indole acetic acid. Correspondingly, it inhibited total soluble sugars, abscisic acid, and lipid peroxidation. Moreover, the application of stigmasterol caused the appearance of new polypeptides and the reappearance of those missed by gamma radiation. Overall, stigmasterol could alleviate the adverse effects of gamma radiation on wheat plants.
电离辐射是一种非生物胁迫，限制了农作物的生长和生产力。豆甾醇对许多作物的生长具有积极作用。豆甾醇在减轻电离辐射对植物代谢和发育影响方面的作用仍不清楚。因此，本研究旨在探讨γ辐射（0、25和50 Gy）预处理、叶面喷施豆甾醇（0、100和200 ppm）及其相互作用对小麦（L.，品种Sids 12）植株生长和生化成分的影响。与对照值相比，25 Gy的γ辐射在株高、根长、叶片数、地上部鲜重、根部鲜重、叶绿素、脱落酸、可溶性酚和丙二醛方面没有显著差异。50 Gy的γ射线抑制了地上部和根部长度、旗叶面积、地上部鲜重和干重、光合色素、总可溶性糖、脯氨酸和过氧化物酶活性。然而，它刺激了总酚、过氧化氢酶活性和脂质过氧化。另一方面，与对照植株相比，100 ppm的豆甾醇对某些生理特性没有显著影响。200 ppm的豆甾醇改善了植物生长参数、光合色素、脯氨酸、酚类、抗氧化酶、赤霉素和吲哚乙酸。相应地，它抑制了总可溶性糖、脱落酸和脂质过氧化。此外，施用豆甾醇导致出现新的多肽，并使γ辐射缺失的多肽重新出现。总体而言，豆甾醇可以减轻γ辐射对小麦植株的不利影响。
