Skliutė Giedrė, Baušytė Raminta, Ramašauskaitė Diana, Navakauskienė Rūta
Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio Av. 7, LT-10257 Vilnius, Lithuania.
Centre of Obstetrics and Gynaecology, Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Santariškiu˛ St., LT-08661 Vilnius, Lithuania.
Biomedicines. 2022 Jun 4;10(6):1324. doi: 10.3390/biomedicines10061324.
Infertility is one of the most rapidly increasing global health concerns of the 21st century. Embryo quality and endometrial thickness and receptivity are the main factors for successful embryo implantation and pregnancy development. Nevertheless, until now, there has been a lack of understanding about the regulation of human endometrium function and its structure. This raises the demand for more research of the human endometrium in these fields. In our study, we analyzed the genetic and epigenetic changes of endometrial tissue's samples isolated from females admitted for treatment due to male infertility and females diagnosed with reproductive pathologies, who are preparing for assisted reproductive technologies procedures. Using real-time polymerase chain reaction method, we demonstrated that endometrium of females with reproductive pathology has significantly upregulated decidualization related genes , , , increased expression of angiogenesis related gene , and increases of overall immune response and inflammation-related genes expression with significant changes of and genes expression. Females with reproductive pathology have altered endometrium epigenetic regulation since expression of miRNAs-specifically, miRNA-34a, miRNA-223, and miRNA-125b-is lower in endometrium of females with reproductive pathology. Our findings suggest that the potential changes in genetic and epigenetic profile of endometrium from females with reproductive pathology could enrich the knowledge in the field of core biological knowledge and treatment of reproductive impairments.
不孕症是21世纪全球范围内迅速增加的主要健康问题之一。胚胎质量、子宫内膜厚度和容受性是胚胎成功着床和妊娠发育的主要因素。然而，迄今为止，人们对人类子宫内膜功能及其结构的调节仍缺乏了解。这就需要在这些领域对人类子宫内膜进行更多研究。在我们的研究中，我们分析了因男性不育症入院治疗的女性以及被诊断患有生殖系统疾病且正准备接受辅助生殖技术治疗的女性的子宫内膜组织样本的基因和表观遗传变化。使用实时聚合酶链反应方法，我们证明患有生殖系统疾病的女性子宫内膜中蜕膜化相关基因、、、的表达显著上调，血管生成相关基因的表达增加，整体免疫反应和炎症相关基因的表达增加，且和基因的表达有显著变化。患有生殖系统疾病的女性子宫内膜的表观遗传调节发生了改变，因为特定的miRNA——miRNA - 34a、miRNA - 223和miRNA - 125b在患有生殖系统疾病的女性子宫内膜中的表达较低。我们的研究结果表明，患有生殖系统疾病的女性子宫内膜基因和表观遗传谱的潜在变化可能会丰富生殖损伤核心生物学知识和治疗领域的知识。
