Pang Sow-Neng, Lin Yu-Lun, Chiou Yueh-Er, Leung Wai-Hung, Weng Wen-Hui
Department of General Medicine, Mater Misericordiae University Hospital, D07 R2WY Dublin, Ireland.
Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan.
Biomedicines. 2022 Jun 17;10(6):1434. doi: 10.3390/biomedicines10061434.
Research in cancer diagnostics has recently established its footing and significance in the biosensor sphere, emphasizing the idea of a unique probe design used as a sensor and actuator, to identify the presence of protein, DNA, RNA, or miRNA. The fluorescein isothiocyanate (FITC) probe and biotinylated probe are designed for a two-pronged approach to the detection of the urinary miR-21 and miR-141, both of which have demonstrated significance in the development and progression of colorectal cancer, a leading cause of mortality and morbidity. The remainder of the apparatus is composed of a modified screen-printed carbon electrode (SPCE), to which the probes adhere, that transduces signals via the redox reaction between H2O2 and HRP, measured with chronoamperometry and cyclic voltammetry. The precise nature of our ultra-non-invasive biosensor makes for a highly sensitive and practical cancer detector, concluded by the significance when establishing disease presence (miR-21 p-value = 0.0176, miR-141 p-value = 0.0032), disease follow-up (miR-21 p-value = 0.00154, miR141 p-value < 0.0005), and even disease severity. This article hopes to emphasize the potential of an additional clinical tool for the management of colorectal cancer.
癌症诊断研究最近在生物传感器领域站稳了脚跟并彰显出其重要性，强调了将独特的探针设计用作传感器和促动器以识别蛋白质、DNA、RNA或miRNA存在的理念。异硫氰酸荧光素（FITC）探针和生物素化探针被设计用于双管齐下检测尿液中的miR-21和miR-141，这两种物质在结直肠癌（一种导致死亡和发病的主要原因）的发展和进展中均已显示出重要意义。该装置的其余部分由修饰的丝网印刷碳电极（SPCE）组成，探针附着于其上，该电极通过H2O2和HRP之间的氧化还原反应传导信号，采用计时电流法和循环伏安法进行测量。我们这种超非侵入性生物传感器的精确特性使其成为一种高度灵敏且实用的癌症探测器，在确定疾病存在（miR-21 p值 = 0.0176，miR-141 p值 = 0.0032）、疾病随访（miR-21 p值 = 0.00154，miR141 p值 < 0.0005）乃至疾病严重程度方面的意义都证实了这一点。本文希望强调一种用于结直肠癌管理的额外临床工具的潜力。
