Chiou Yueh-Er, Yu Kai-Jie, Pang Sow-Neng, Yang Yan-Lin, Pang See-Tong, Weng Wen-Hui
Department of Nursing, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan.
Bioengineering (Basel). 2022 Dec 14;9(12):803. doi: 10.3390/bioengineering9120803.
Prostate cancer is the most common cancer in the male population, carrying a significant disease burden. PSA is a widely available screening tools for this disease. Current screen-printed carbon electrode (SPCE)-based biosensors use a two-pronged probe approach to capture urinary miRNA. We were able to successfully detect specific exosomal miRNAs (exomiRs) in the urine of patients with prostate cancer, including exomiR-451 and exomiR-21, and used electrochemistry for measurement and analysis. Our results significantly reaffirmed the presence of exomiR-451 in urine and that a CV value higher than 220 nA is capable of identifying the presence of disease (-value = 0.005). Similar results were further proven by a PAS greater than 4 (-value = 0.001). Moreover, a higher urinary exomiR-21 was observed in the high-T3b stage; this significantly decreased following tumor removal (-values were 0.016 and 0.907, respectively). According to analysis of the correlation with tumor metastasis, a higher exomiR-21 was associated with lymphatic metastasis (p-value 0.042), and higher exomiR-461 expression was correlated with tumor stage (-value 0.031), demonstrating that the present exomiR biosensor can usefully predict tumor progression. In conclusion, this biosensor represents an easy-to-use, non-invasive screening tool that is both sensitive and specific. We strongly believe that this can be used in conjunction with PSA for the screening of prostate cancer.
前列腺癌是男性群体中最常见的癌症，带来了重大的疾病负担。PSA是用于该疾病的一种广泛可用的筛查工具。当前基于丝网印刷碳电极（SPCE）的生物传感器采用双叉探针方法来捕获尿液中的微小RNA。我们能够成功检测前列腺癌患者尿液中的特定外泌体微小RNA（外泌体miRNA），包括外泌体miR - 451和外泌体miR - 21，并用电化学方法进行测量和分析。我们的结果显著地再次证实了尿液中外泌体miR - 451的存在，并且循环伏安（CV）值高于220 nA能够识别疾病的存在（P值 = 0.005）。大于4的阳性似然比（PAS）进一步证明了类似结果（P值 = 0.001）。此外，在高T3b期观察到尿液中外泌体miR - 21水平更高；肿瘤切除后该水平显著下降（P值分别为0.016和0.907）。根据与肿瘤转移的相关性分析，较高的外泌体miR - 21与淋巴转移相关（P值0.042），并且较高的外泌体miR - 461表达与肿瘤分期相关（P值0.031），这表明当前的外泌体miRNA生物传感器可有效预测肿瘤进展。总之，这种生物传感器是一种易于使用、非侵入性的筛查工具，兼具灵敏性和特异性。我们坚信它可与PSA联合用于前列腺癌的筛查。
