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接合型大肠杆菌中的细胞间相互作用：F菌毛的作用及交配聚集体的命运

Cell-cell interactions in conjugating Escherichia coli: role of F pili and fate of mating aggregates.

作者信息

Achtman M, Morelli G, Schwuchow S

出版信息

J Bacteriol. 1978 Sep;135(3):1053-61. doi: 10.1128/jb.135.3.1053-1061.1978.

DOI:10.1128/jb.135.3.1053-1061.1978
PMID:357413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC222482/
Abstract

Bacterial conjugation between Escherichia coli cells was investigated by a combination of physical and genetic techniques, using Hfr, F', or R+ donors and F- recipients. DNA transfer occurred in mating aggregates of up to 50 cells. Multiple interactions between donor and recipient cells occurred, and both F- pilus connections and wall-to-wall contacts were detectable. The detectable F- pilus contacts could be destroyed without either disrupting the mating aggregates or preventing DNA transfer. Hfr X F- mating aggregates did not disaggregate even though recombinant frequencies were inversely proportional to the distance from the origin of DNA transfer. F' or R+ donors formed mating aggregates with F- cells which disaggregated soon after transfer of the autonomous sex factor DNA.

摘要

利用高频重组（Hfr）、F'或R+供体菌以及F-受体菌，通过物理和遗传技术相结合的方法研究了大肠杆菌细胞间的细菌接合作用。DNA转移发生在多达50个细胞的交配聚集体中。供体细胞与受体细胞之间发生了多次相互作用，并且可以检测到F-菌毛连接以及壁对壁接触。可检测到的F-菌毛接触可以被破坏，而不会破坏交配聚集体或阻止DNA转移。即使重组频率与DNA转移起点的距离成反比，Hfr×F-交配聚集体也不会解体。F'或R+供体菌与F-细胞形成交配聚集体，在自主性别因子DNA转移后不久就会解体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f2/222482/326d1ec2e4a5/jbacter00292-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f2/222482/326d1ec2e4a5/jbacter00292-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f2/222482/326d1ec2e4a5/jbacter00292-0335-a.jpg

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本文引用的文献

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A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION.一种快速有效的细菌接合阻断方法。
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Two pKM101-encoded proteins, the pilus-tip protein TraC and Pep, assemble on the Escherichia coli cell surface as adhesins required for efficient conjugative DNA transfer.两个 pKM101 编码蛋白，菌毛尖端蛋白 TraC 和 Pep，作为大肠杆菌细胞表面上用于有效接合性 DNA 转移的黏附素组装。
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