Masilionyte Ugne, Gedvilaite Greta, Kaikaryte Kriste, Vilkeviciute Alvita, Kriauciuniene Loresa, Glebauskiene Brigita, Balnyte Renata, Liutkeviciene Rasa
Medical Academy, Lithuanian University of Health Sciences, Eiveniu Street 2, LT-50161 Kaunas, Lithuania.
Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Eiveniu Street 2, LT-50161 Kaunas, Lithuania.
Brain Sci. 2022 Jun 18;12(6):800. doi: 10.3390/brainsci12060800.
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system with features of demyelination and axonal degeneration at a young age. Genetic factors may play an important role in the development of multiple sclerosis. (1) Objective: To investigate IL-10 rs1800871, rs1800872, rs1800896, and IL-10 serum levels in patients with multiple sclerosis. (2) Methods: Our study included patients with multiple sclerosis (n = 127) and healthy volunteers (n = 195). The subjects’ DNA was extracted from peripheral blood leukocytes and genotyped by real-time polymerase chain reaction. The results were analyzed using the program “IBM SPSS Statistics 27.0”. (3) Results: The IL-10 SNPs were analyzed between the MS and control groups; however, no statistically significant results were found. The serum levels of IL-10 in the groups of MS and healthy subjects were not statistically significantly different (median (IQR): 0.828 (1.533) vs. 0.756 (0.528), p = 0.872). (4) Conclusions: IL-10 rs1800871, rs1800872, and rs1800896 and serum IL-10 levels are not likely to be associated with MS development. However, individuals carrying the rare haplotypes of rs1800871, rs1800872, and rs1800896 were associated with increased odds of MS (p = 0.006).
多发性硬化症（MS）是一种中枢神经系统的慢性炎症性疾病，在年轻时具有脱髓鞘和轴突变性的特征。遗传因素可能在多发性硬化症的发展中起重要作用。（1）目的：研究多发性硬化症患者中白细胞介素-10（IL-10）的rs1800871、rs1800872、rs1800896基因位点及IL-10血清水平。（2）方法：我们的研究纳入了多发性硬化症患者（n = 127）和健康志愿者（n = 195）。从外周血白细胞中提取受试者的DNA，并通过实时聚合酶链反应进行基因分型。使用“IBM SPSS Statistics 27.0”程序分析结果。（3）结果：对MS组和对照组之间的IL-10单核苷酸多态性（SNP）进行了分析；然而，未发现具有统计学意义的结果。MS组和健康受试者组的IL-10血清水平无统计学显著差异（中位数（四分位间距）：0.828（1.533）对0.756（0.528），p = 0.872）。（4）结论：IL-10的rs1800871、rs1800872和rs1800896基因位点及血清IL-10水平不太可能与MS的发生相关。然而，携带rs1800871、rs1800872和rs1800896罕见单倍型的个体与MS发病几率增加相关（p = 0.006）。
