State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of the P. R. China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of the P. R. China, Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2022 May 31;13(6):988. doi: 10.3390/genes13060988.
Non-heading Chinese cabbage ( ssp. ) is an important vegetative crop in the south of China. As an antioxidant, anthocyanin is the major quality trait for vegetables with purple leaves or petioles. However, the molecular biosynthetic mechanism of anthocyanin in non-heading Chinese cabbage has not been explained exclusively. In this study, two non-heading Chinese cabbage with contrasting colors in the leaves were used as the materials for RNA-seq. A total of 906 DEGs were detected, and we found that the anthocyanin and flavonoid biosynthetic pathways are significantly enriched in the purple NHCC. The transcriptome result was verified by RT-qPCR. Though bioinformatics analysis, was selected as the candidate gene for the regulation of anthocyanin synthesis, and the characterization of was elucidated by the functional analyses. The results proved that BcTT8 is a nucleus protein and phylogenetically close to the TT8 protein from . After silencing , the total anthocyanin content of pTY- plants decreased by 42.5%, and the relative expression levels of anthocyanin pathway genes , and were significantly downregulated, while the transcription level of was significantly upregulated. Compared with the wild type, the transgenic showed obvious violet in the cotyledons part, and the anthocyanin biosynthetic genes such as and were significantly upregulated. In conclusion, is critical in the anthocyanin synthesis process of non-heading Chinese cabbage. Our findings illustrated the molecular mechanism of anthocyanin biosynthesis in non-heading Chinese cabbage.
不结球白菜（ ssp. ）是中国南方重要的叶菜类蔬菜。作为一种抗氧化剂，花色苷是叶片或叶柄呈紫色蔬菜的主要品质特性。然而，不结球白菜花色苷的分子生物合成机制尚未完全阐明。本研究以叶片颜色存在显著差异的两个不结球白菜品种为材料进行 RNA-seq 分析。共检测到 906 个差异表达基因，发现花色苷和类黄酮生物合成途径在紫色 NHCC 中显著富集。通过 RT-qPCR 验证了转录组结果。通过生物信息学分析，选择 作为调控花色苷合成的候选基因，并通过功能分析阐明了 的特征。结果表明，BcTT8 是一种核蛋白，与 中的 TT8 蛋白在系统发育上亲缘关系较近。沉默 后，pTY- 植株的总花色苷含量下降了 42.5%，花色苷途径基因 、 和 的相对表达水平显著下调，而 的转录水平显著上调。与野生型相比，转基因 子叶部分呈明显的紫罗兰色，花色苷生物合成基因 、 和 等显著上调。综上所述， 对不结球白菜花色苷合成至关重要。本研究结果阐明了不结球白菜花色苷生物合成的分子机制。
