Fu Mei, Guo Juxian, Tang Kang, Jiang Shizheng, Luo Shanwei, Luo Wenlong, Khan Imran, Li Guihua
Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China.
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2023 Sep 11;24(18):13951. doi: 10.3390/ijms241813951.
Flowering Chinese cabbage ( var. ) is one of the most popular vegetables in the south of China. As an antioxidant, anthocyanin is an important quality trait in vegetables, and the gene related to anthocyanin biosynthesis in purple flowering Chinese cabbage is also important. In this study, two flowering Chinese cabbage with extreme colors in the stem were used as materials for transcriptome analysis. RNA-seq analysis showed that 6811 differentially expressed genes (DEGs) were identified, including 295 transcription factors. Phenylpropanoid biosynthesis, flavone and flavanol biosynthesis, and flavonoid biosynthesis pathways were found to be significantly enriched in the purple flowering Chinese cabbage. A total of 25 DEGs associated with anthocyanin biosynthesis were found at a higher expression in purple flowering Chinese cabbage than in green flowering Chinese cabbage. Bioinformatics analysis shows that is a candidate gene for the regulation of anthocyanin biosynthesis, and heterologous expression analysis of in indicates that functions in anthocyanin biosynthesis. Therefore, our findings provide vital evidence for elucidating the molecular mechanism in the purple stem in flowering Chinese cabbage.
菜心（变种）是中国南方最受欢迎的蔬菜之一。花青素作为一种抗氧化剂，是蔬菜中的重要品质性状，紫色菜心中与花青素生物合成相关的基因也很重要。在本研究中，选取了两种茎部颜色差异极大的菜心作为转录组分析材料。RNA测序分析表明，共鉴定出6811个差异表达基因（DEG），其中包括295个转录因子。发现苯丙烷生物合成、黄酮和黄烷醇生物合成以及类黄酮生物合成途径在紫色菜心中显著富集。共发现25个与花青素生物合成相关的差异表达基因在紫色菜心中的表达高于绿色菜心。生物信息学分析表明，[此处原文缺失具体基因名称]是调控花青素生物合成的候选基因，在[此处原文缺失具体受体]中的异源表达分析表明，[此处原文缺失具体基因名称]在花青素生物合成中发挥作用。因此，我们的研究结果为阐明菜心紫色茎的分子机制提供了重要证据。
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