Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
Sci Rep. 2022 Apr 15;12(1):6308. doi: 10.1038/s41598-022-10106-1.
Chinese cabbage that prefers cold conditions is also affected by low-temperature stress, such as the accumulation of leaf anthocyanins. Research on anthocyanin biosynthesis and regulation mechanisms has made great progress. However, research on anthocyanin accumulation for resistance to biological and non-biological stress is still lacking. To study the relationship between anthocyanin accumulation of Chinese cabbage and resistance under low-temperature conditions, RNA sequencing (RNA-seq) was performed on Chinese cabbage 'Xiao Baojian' grown at a low temperature for four time periods and at a control temperature for five time periods. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, 7954 differentially expressed genes (DEGs) were enriched, of which 587 DEGs belonged to "biosynthesis of other secondary metabolites." Gene temporal expression patterns were used to discover enriched genes related to phenylpropanoid biosynthesis; flavonoid biosynthesis and anthocyanin biosynthesis pathways were found in cluster 1. The interaction networks were constructed, and hub genes were selected, showing that flavonoid biosynthesis pathway genes (DFR, ANS, F3H, FLS1, CHS1, CHS3, and TT8) and defense mechanisms-related genes (DFR, SNL6, and TKPR1) interact with each other. Anthocyanin biosynthesis DEGs in Chinese cabbage were evaluated under low-temperature conditions to map the relevant pathways, and expression maps of transcription factors in the flavonoid pathway were created at various periods. Low temperature upregulated the expression of genes related to anthocyanin biosynthesis. Taken together, our results provide further analysis of the relationship between plant anthocyanin synthesis and stress resistance and may also provide further insights for the future development of high-quality color and cold-tolerant Chinese cabbage germplasm resources.
喜欢冷凉条件的白菜也会受到低温胁迫的影响，如叶片花色素苷的积累。关于花色素苷生物合成和调控机制的研究已经取得了很大进展。然而，关于花色素苷积累对生物和非生物胁迫的抗性的研究仍然缺乏。为了研究白菜在低温条件下的花色素苷积累与抗性的关系，对在低温下生长 4 个时期和在对照温度下生长 5 个时期的“小保剑”白菜进行了 RNA 测序（RNA-seq）。在京都基因与基因组百科全书（KEGG）途径中，有 7954 个差异表达基因（DEGs）被富集，其中 587 个 DEGs 属于“次生代谢物的其他生物合成”。基因时间表达模式用于发现与苯丙烷生物合成相关的富集基因；在聚类 1 中发现了类黄酮生物合成和花色素苷生物合成途径。构建了互作网络，并选择了枢纽基因，显示类黄酮生物合成途径基因（DFR、ANS、F3H、FLS1、CHS1、CHS3 和 TT8）和防御机制相关基因（DFR、SNL6 和 TKPR1）相互作用。评估了低温条件下白菜中花色素苷生物合成的 DEGs，以绘制相关途径，并创建了不同时期黄酮途径转录因子的表达图谱。低温上调了与花色素苷生物合成相关的基因的表达。总之，我们的研究结果为植物花色素苷合成与抗逆性的关系提供了进一步的分析，也可能为未来高品质花色和耐冷白菜种质资源的开发提供进一步的思路。
