Shin Sarah, Kim Ji Soo, Park Myung Ku, Bang Ok-Sun
KM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea.
Korea Testing & Research Institute, Hwasun 58141, Korea.
Foods. 2022 Jun 16;11(12):1773. doi: 10.3390/foods11121773.
This study aimed to evaluate the genotoxic potential of the fruit and seed powder, fruit puree, and aqueous extracts of ( noni). The genotoxic potential of the noni substances was evaluated using in vitro Ames, in vitro chromosomal aberration, and in vivo micronucleus tests. All test procedures were conducted per Organization for Economic Cooperation and Development guidelines, and good laboratory practice. None of the noni fruit test substances showed genotoxic signs up to 5000 and 2000 μg/plate in the Ames and micronucleus tests, respectively. In the chromosomal aberration test, neither the fruit puree nor aqueous extract showed structural and numerical aberrations up to 5000 and 4650 μg/mL, respectively, irrespective of metabolic activation, in both 6 h and 24 h treatment groups. The safe ranges of noni fruit and seed powders were up to 2500 and 2100 μg/mL, respectively, in the 6 h treatment group and up to 1600-4100 and 370-450 μg/mL, respectively, in both 6 h and 24 h treatment groups in the presence of metabolic activation. Noni fruit and seeds were safe in terms of genotoxicity under our experimental conditions. Our data are the first to provide valuable genotoxic information on .
本研究旨在评估海巴戟（诺丽）果实和种子粉末、果泥及水提取物的遗传毒性潜力。使用体外艾姆斯试验、体外染色体畸变试验和体内微核试验评估诺丽物质的遗传毒性潜力。所有试验程序均按照经济合作与发展组织的指南及良好实验室规范进行。在艾姆斯试验和微核试验中，诺丽果实受试物质分别在高达5000和2000μg/平板时均未显示出遗传毒性迹象。在染色体畸变试验中，无论有无代谢活化，果泥和水提取物在6小时和24小时处理组中，分别在高达5000和4650μg/mL时均未显示出结构和数量畸变。在6小时处理组中，诺丽果实和种子粉末的安全范围分别高达2500和2100μg/mL；在有代谢活化的情况下，在6小时和24小时处理组中，安全范围分别高达1600 - 4100和370 - 450μg/mL。在我们的实验条件下，诺丽果实和种子在遗传毒性方面是安全的。我们的数据首次提供了关于海巴戟的有价值的遗传毒性信息。
