Kustiarini Dyah Aninta, Nishigaki Toshiaki, Kanno Hiroyuki, To Hideto
Department of Medical Pharmaceutics, Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama.
Centre for Agro-Based Industry.
Biol Pharm Bull. 2019;42(3):496-500. doi: 10.1248/bpb.b18-00480.
Morinda citrifolia L., known as noni, originated from Indonesia exhibits various pharmacological activities including anti-inflammatory properties. However, the validity of noni fruit juice as a treatment for rheumatoid arthritis (RA), an autoimmune disorder, has not been confirmed yet. Therefore, the main purpose of this research was to evaluate the efficacy of noni fruit juice (INFJ) made in Indonesia using SKG mice as an animal model of RA, which shows the resembling characteristics of human RA patients. Furthermore, the safety of INFJ was examined by repeated dose experiments in mice. INFJ was mixed with water at 50% and administered to SKG mice sensitized with mannan, free access for 4 weeks. Arthritis scores of fore- and hind-leg joints were measured and the joints were histopathologically examined. The sub-acute and sub-chronic toxicities of INFJ were evaluated using BALB/c mice. The arthritic scores were significantly lower from the 7 d after sensitization in the INFJ group than the control group. Histopathological examinations of the joints revealed inhibition of severity of RA. In both toxicity studies, INFJ did not show any toxicities. INFJ exhibited anti-arthritic activity in arthritic and histopathological examinations of the joints in SKG mice. Present study was the first report where noni juice may be effective against RA. The dose of noni juice showing efficacy against RA was confirmed safe from repeated dose studies in mice.
海巴戟天，又称诺丽果，原产于印度尼西亚，具有多种药理活性，包括抗炎特性。然而，诺丽果汁作为一种治疗类风湿性关节炎（RA）（一种自身免疫性疾病）的疗法的有效性尚未得到证实。因此，本研究的主要目的是使用SKG小鼠作为RA动物模型来评估印度尼西亚生产的诺丽果汁（INFJ）的疗效，该模型显示出与人类RA患者相似的特征。此外，通过在小鼠中进行重复剂量实验来检测INFJ的安全性。将INFJ与50%的水混合，给予用甘露聚糖致敏的SKG小鼠，自由饮用4周。测量前肢和后肢关节的关节炎评分，并对关节进行组织病理学检查。使用BALB/c小鼠评估INFJ的亚急性和亚慢性毒性。INFJ组致敏后7天的关节炎评分显著低于对照组。关节的组织病理学检查显示RA严重程度受到抑制。在两项毒性研究中，INFJ均未显示出任何毒性。在SKG小鼠的关节关节炎和组织病理学检查中，INFJ表现出抗关节炎活性。本研究是关于诺丽果汁可能有效对抗RA的首次报道。从对小鼠的重复剂量研究中证实，显示出对抗RA疗效的诺丽果汁剂量是安全的。
