Guangdong Provincial Key Laboratory of Applied Botany and South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
CAS Engineering Laboratory for Vegetation Ecosystem Restoration on Islands and Coastal Zones, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Int J Mol Sci. 2022 Jun 8;23(12):6405. doi: 10.3390/ijms23126405.
Heat shock protein 20 (Hsp20) is a major family of heat shock proteins that mainly function as molecular chaperones and are markedly accumulated in cells when organisms are subjected to environmental stress, particularly heat. is an extremophile halophyte with good adaptability to environmental high temperature and is widely distributed in coastal areas or islands in tropical and subtropical regions. In this study, we identified a total of 41 genes in the genome. The gene structures, phylogenetic relationships, chromosome locations, and conserved motifs of each or encoding protein were analyzed. The promoters of s contained a series of predicted -acting elements, which indicates that the expression of different members is regulated precisely. The expression patterns of the family were analyzed by RNA sequencing both at the tissue-specific level and under different abiotic stresses, and were further validated by quantitative reverse transcription PCR. The integrated expression profiles of the s indicated that most genes were greatly upregulated (up to dozens to thousands of times) after 2 h of heat stress. However, some of the heat-upregulated genes showed completely different expression patterns in response to salt, alkaline, or high osmotic stresses, which indicates their potential specific function in mediating the response of to abiotic stresses. In addition, some of s were cloned and functionally characterized for their roles in abiotic stress tolerance in yeast. Taken together, these findings provide a foundation for functionally characterizing s to unravel their possible roles in the adaptation of this species to tropical coral reefs. Our results also contribute to the understanding of the complexity of the response of genes to other abiotic stresses and may help in future studies evaluating the functional characteristics of s for crop genetic improvement.
热休克蛋白 20（Hsp20）是热休克蛋白大家族中的主要成员，主要作为分子伴侣发挥作用，当生物体受到环境压力，尤其是热压力时，其在细胞中的积累量显著增加。是一种嗜盐的盐生植物，对环境高温有很好的适应性，广泛分布于热带和亚热带地区的沿海地区或岛屿。在本研究中，我们从 基因组中共鉴定出 41 个 基因。分析了每个 或编码蛋白的基因结构、系统进化关系、染色体定位和保守基序。 基因启动子含有一系列预测的 - 作用元件，这表明不同 成员的表达受到精确调控。通过 RNA 测序分析了 家族在组织特异性水平和不同非生物胁迫下的表达模式，并通过定量反转录 PCR 进一步验证。 家族的综合表达谱分析表明，大多数 基因在热胁迫 2 小时后被强烈上调（高达数十到数千倍）。然而，一些受热上调的 基因在响应盐、碱性或高渗胁迫时表现出完全不同的表达模式，这表明它们在介导 对非生物胁迫的反应中具有潜在的特定功能。此外，还克隆和功能表征了一些 基因，以研究它们在酵母非生物胁迫耐受中的作用。综上所述，这些发现为功能表征 基因提供了基础，以揭示它们在该物种适应热带珊瑚礁中的可能作用。我们的研究结果还有助于理解 基因对其他非生物胁迫的反应的复杂性，并可能有助于未来研究评估 基因在作物遗传改良中的功能特征。
