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蛋白质组学和磷酸化蛋白质组学分析揭示了调控花粉败育的复杂网络和 TCMS 小麦中的潜在候选蛋白。

Proteomic and Phosphoproteomic Analyses Reveal a Complex Network Regulating Pollen Abortion and Potential Candidate Proteins in TCMS Wheat.

机构信息

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Horticulture, Northwest A&F University, Yangling 712100, China.

出版信息

Int J Mol Sci. 2022 Jun 8;23(12):6428. doi: 10.3390/ijms23126428.

DOI:10.3390/ijms23126428
PMID:35742874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9224247/
Abstract

Thermosensitive sterile lines are natural materials for exploring the effects of anther development on male fertility. To study the possible molecular mechanisms regulating protein activity during the induction of male sterility, proteomic and phosphoproteomic analyses with tandem mass tags (TMTs) were used to study the binucleate anther of the thermosensitive sterile wheat line YS3038. A total of 9072 proteins, including 5019 phosphoproteins, were identified. Enrichment analyses of differentially abundant proteins (DAPs) and phosphoproteins (DAPPs) in metabolic pathways showed that both were mainly related to energy metabolism. Soluble sugar and ATP content were significantly decreased, free fatty acid content was significantly increased, and ROS was abnormally accumulated in male sterile YS3038-A. In addition, 233 kinase-substrate pairs involved in potential phosphorylation control networks were predicted to regulate fertility. Candidate proteins were identified, and a quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to validate the TMT results. is likely to be involved in fertility conversion of YS3038 by barley stripe mosaic virus-induced gene silencing (BSMV-VIGS). Our data provide new insights into the mechanism of TCMS, which has value for identifying potential candidate proteins associated with the formation or abortion of pollen and promotion of wheat heterosis utilization.

摘要

热不稳定不育系是探索花粉发育对雄性育性影响的天然材料。为了研究诱导雄性不育过程中调节蛋白质活性的可能分子机制，使用串联质量标签 (TMT) 进行蛋白质组学和磷酸化蛋白质组学分析，以研究热敏不育小麦系 YS3038 的双核花药。共鉴定出 9072 种蛋白质，包括 5019 种磷酸化蛋白质。差异丰度蛋白质 (DAP) 和磷酸化蛋白质 (DAPP) 在代谢途径中的富集分析表明，它们主要与能量代谢有关。雄性不育 YS3038-A 中的可溶性糖和 ATP 含量显著降低，游离脂肪酸含量显著增加，ROS 异常积累。此外，预测了 233 个参与潜在磷酸化调控网络的激酶-底物对，以调节育性。鉴定候选蛋白，并使用实时定量聚合酶链反应 (qRT-PCR) 分析验证 TMT 结果。 可能通过大麦黄花叶病毒诱导的基因沉默 (BSMV-VIGS) 参与 YS3038 的育性转换。我们的数据为 TCMS 的机制提供了新的见解，这对于鉴定与花粉形成或败育以及促进小麦杂种优势利用相关的潜在候选蛋白具有重要价值。

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