Laboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, Japan.
Int J Mol Sci. 2022 Jun 10;23(12):6488. doi: 10.3390/ijms23126488.
Neurosecretory protein GL (NPGL) is a small secretory protein identified in the hypothalamus of birds and mammals. We recently reported that NPGL exerts obesogenic effects in obesity-prone C57BL6/J mice. However, whether NPGL elicits adiposity in different mouse strains is poorly understood. In this study, we generated transgenic mice overexpressing using the ICR strain ( Tg mice) to elucidate the obesogenic effects of NPGL in different strains. Tg mice showed increased white adipose tissue (WAT) mass. Although the mass of brown adipose tissue (BAT) was slightly altered in Tg mice, hypertrophy of lipid droplets was also observed in BAT. In contrast, fat accumulation was not induced in the liver, with the upregulation of mRNAs related to hepatic lipolysis. These results support the hypothesis that NPGL causes obesity in several strains and species. This report highlights the pivotal role of NPGL in fat accumulation in adipose tissues and contributes to the elucidation of the biological mechanisms underlying obesity and metabolic diseases in heterogeneous populations.
神经分泌蛋白 GL（NPGL）是一种在鸟类和哺乳动物的下丘脑发现的小分泌蛋白。我们最近报道，NPGL 在肥胖易感的 C57BL6/J 小鼠中发挥致肥胖作用。然而，NPGL 是否在不同的小鼠品系中引起肥胖尚不清楚。在这项研究中，我们使用 ICR 品系（Tg 小鼠）生成了过表达 NPGL 的转基因小鼠，以阐明 NPGL 在不同品系中的致肥胖作用。Tg 小鼠表现出白色脂肪组织（WAT）质量增加。尽管 Tg 小鼠的棕色脂肪组织（BAT）质量略有改变，但也观察到 BAT 中脂质滴的肥大。相反，肝脏中没有诱导脂肪堆积，与肝脂解相关的 mRNA 上调。这些结果支持 NPGL 在几种品系和物种中引起肥胖的假说。本报告强调了 NPGL 在脂肪组织中脂肪积累中的关键作用，并有助于阐明异质人群中肥胖和代谢性疾病的生物学机制。
