Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.
Amino Acids. 2023 Nov;55(11):1557-1562. doi: 10.1007/s00726-023-03328-8. Epub 2023 Sep 9.
Our recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in vivo studies will be required to confirm this.
我们最近的研究证实，成熟的神经肽 FAM237A，也被称为神经分泌蛋白 GL（NPGL），是 GPR83 的有效激动剂。先前报道的同源物 FAM237B 是 GPR83 的弱激动剂。在本研究中，我们通过内含肽融合方法制备了成熟的人源 FAM237B，并证明它可以在基于 NanoBiT 的β-arrestin 招募测定中在纳摩尔范围内（1‒10 nM）引起显著的激活效应。因此，FAM237B 似乎是 GPR83 的另一种内源性激动剂，需要进行未来的体内研究来证实这一点。
