Biology Department, La Sierra University, Riverside, CA 92505, USA.
Int J Mol Sci. 2022 Jun 14;23(12):6609. doi: 10.3390/ijms23126609.
is a Gram-positive bacteria found in organic matter. Three lytic bacteriophages, KaiHaiDragon, OneinaGillian, and YuuY, were isolated from strain NRRL B-24224. Phage YuuY in particular expresses a broad host range as it possesses the ability to infect closely related bacterial species at a high plating efficiency. Characterization tests were performed on all three phage to assess morphology, genomic characteristics, pH and thermal stabilities, life cycle, and the type of receptor used for infection. All three phages showed similar pH stability, ranging from pH 5-11, except for KaiHaiDragon, which had a reduced infection effectiveness at a pH of 11. YuuY possessed a significantly higher temperature tolerance compared to the other phages as some phage particles remained viable after incubation temperatures of up to 80 °C. Based on the one-step growth curve assay, all three phages possessed a relatively short latent period of 90 min and an approximately two-fold burst size factor. Moreover, all three phages utilize a carbohydrate receptor to initiate infection. Based on bioinformatics analysis, YuuY, KaiHaiDragon and OneinaGillian were assigned to clusters EA10, EC, and EG, respectively.
是一种存在于有机物中的革兰氏阳性细菌。三种溶菌噬菌体 KaiHaiDragon、OneinaGillian 和 YuuY 从 NRRL B-24224 菌株中分离出来。噬菌体 YuuY 特别表达了广泛的宿主范围，因为它具有以高平板效率感染密切相关细菌物种的能力。对所有三种噬菌体进行了特征描述测试，以评估形态、基因组特征、pH 和热稳定性、生命周期以及用于感染的受体类型。所有三种噬菌体的 pH 稳定性相似，范围为 pH 5-11，除了 KaiHaiDragon，其在 pH 11 时感染效果降低。与其他噬菌体相比，YuuY 具有更高的温度耐受性，因为一些噬菌体颗粒在高达 80°C 的孵育温度下仍然存活。根据一步生长曲线测定，三种噬菌体都具有相对较短的潜伏期 90 分钟和约两倍的爆发大小因子。此外，所有三种噬菌体都利用碳水化合物受体来启动感染。根据生物信息学分析，YuuY、KaiHaiDragon 和 OneinaGillian 分别被分配到聚类 EA10、EC 和 EG 中。
