Department of Pharmaceutical Technology, Medical University of Białystok, Mickiewicza 2c, 15-222 Białystok, Poland.
Department of Physical Chemistry, Faculty of Chemistry, University of Białystok, Ciołkowskiego 1K, 15-245 Białystok, Poland.
Int J Mol Sci. 2022 Jun 17;23(12):6775. doi: 10.3390/ijms23126775.
Hydrogels are semi-solid systems with high flexibility, which, due to holding large amounts of water, are similar to natural tissues and are very useful in the field of biomedical applications. Despite the wide range of polymers available to form hydrogels, novel techniques utilized to obtain hydrogels with adequate properties are still being developed. The aim of this study was to evaluate the impact of the freeze-thaw technique on the properties of cryogels based on sodium alginate and chitosan glutamate with posaconazole as a model antifungal substance. The effect of the freezing and thawing process on the physicochemical, rheological, textural and bioadhesive properties of prepared cryogels was examined. Additionally, the antifungal activity against , and of designed formulations was examined. It was shown that the freeze-thaw technique significantly improved viscosity, bioadhesiveness, textural properties and prolonged the in vitro posaconazole release. Moreover, alginate/chitosan glutamate cryogels exhibited higher values of inhibition zone in culture than traditional hydrogel formulations.
水凝胶是一种具有高柔韧性的半固态系统，由于其含有大量水分，因此类似于天然组织，在生物医学应用领域非常有用。尽管有多种聚合物可用于形成水凝胶，但仍在开发用于获得具有适当性能的水凝胶的新技术。本研究旨在评估冷冻-解冻技术对基于海藻酸钠和壳聚糖谷氨酸的冷冻凝胶特性的影响，其中泊沙康唑作为模型抗真菌物质。研究了冷冻和解冻过程对所制备的冷冻凝胶的物理化学、流变学、质构和生物黏附特性的影响。此外，还研究了设计配方对 、 和 的抗真菌活性。结果表明，冷冻-解冻技术显著提高了黏度、生物黏附性、质构特性，并延长了泊沙康唑的体外释放。此外，与传统水凝胶配方相比，海藻酸钠/壳聚糖谷氨酸冷冻凝胶在 培养物中显示出更高的抑菌圈值。
