抗氧化剂会抑制维生素C对氧自由基诱导的姐妹染色单体交换的作用。

Antioxidants inhibit the effect of vitamin C on oxygen radical-induced sister-chromatid exchanges.

作者信息

Weitberg A B

出版信息

Mutat Res. 1987 May;191(1):53-6. doi: 10.1016/0165-7992(87)90170-9.

Abstract

The mechanism of vitamin C-induced sister-chromatid exchanges in cultured mammalian cells was studied. Chinese hamster ovary cells, when exposed to an enzymatic oxygen radical-generating system (xanthine oxidase plus hypoxanthine), develop increased numbers of sister-chromatid exchanges. Inclusion of ascorbate (greater than or equal to 0.1 mM) in these incubations resulted in an augmentation of this effect. Superoxide dismutase (100 microliter/ml) and catalase (220 microliter/ml) caused a significant reduction in the number of sister-chromatid exchanges induced by xanthine oxidase, hypoxanthine and vitamin C. Their heat-inactivated counterparts had no effect. These results confirm that vitamin C (greater than or equal to 0.1 mM) potentiates the genetic toxicity of oxygen radicals and that this effect is mediated by toxic oxygen intermediates.

摘要

对维生素C诱导培养的哺乳动物细胞姐妹染色单体交换的机制进行了研究。中国仓鼠卵巢细胞在暴露于酶促产生活性氧自由基的系统（黄嘌呤氧化酶加次黄嘌呤）时，姐妹染色单体交换的数量会增加。在这些孵育体系中加入抗坏血酸盐（≥0.1 mM）会增强这种效应。超氧化物歧化酶（100微升/毫升）和过氧化氢酶（220微升/毫升）能显著减少由黄嘌呤氧化酶、次黄嘌呤和维生素C诱导的姐妹染色单体交换的数量。它们经热灭活的对应物则没有作用。这些结果证实，维生素C（≥0.1 mM）可增强氧自由基的遗传毒性，且这种效应是由有毒的氧中间体介导的。