Weitberg A B, Weitzman S A
Mutat Res. 1985 Sep;144(1):23-6. doi: 10.1016/0165-7992(85)90119-8.
We studied the effects of vitamin C (sodium ascorbate) on the genotoxicity of oxygen radicals to tissue culture cells. Chinese hamster ovary cells (CHO cells), when exposed to an enzymatic oxygen radical generating system (xanthine oxidase plus hypoxanthine), develop increased numbers of sister-chromatid exchanges (SCEs). Inclusion of ascorbate in these incubations resulted in significant, but variable effects. In some cases, ascorbate (less than 0.1 mM) was protective and fewer SCEs were produced. In others, significant augmentation of oxygen radical-induced SCEs occurred. These experiments illustrate the complexity of the interactions of ascorbate in biologic systems and the difficulty of predicting a desirable or harmful effect.
我们研究了维生素C（抗坏血酸钠）对氧自由基对组织培养细胞遗传毒性的影响。中国仓鼠卵巢细胞（CHO细胞）在暴露于酶促氧自由基生成系统（黄嘌呤氧化酶加次黄嘌呤）时，姐妹染色单体交换（SCE）的数量会增加。在这些培养物中加入抗坏血酸会产生显著但可变的影响。在某些情况下，抗坏血酸（小于0.1 mM）具有保护作用，产生的SCE较少。在其他情况下，则会显著增强氧自由基诱导的SCE。这些实验说明了抗坏血酸在生物系统中相互作用的复杂性，以及预测其有益或有害影响的难度。
