Department of Physiology, School of Medicine, Showa University, Tokyo 142-8555, Japan.
Department of Urology, School of Medicine, Showa University, Tokyo 142-8666, Japan.
Medicina (Kaunas). 2022 Jun 15;58(6):810. doi: 10.3390/medicina58060810.
: The Japanese herbal medicine Yokukansan (YKS) has analgesic properties and is used for various pain disorders. The purpose of the present study was to investigate the effects of YKS in Hunner-type interstitial cystitis (HIC) using an experimental rat model of HIC and to explore its antioxidant activity and role as the underlying mechanism of action. : The antioxidant capacity of YKS was evaluated by determining its hydroxyl radical (·OH) scavenging capacity using electron spin resonance (ESR). Next, the effects of YKS administration were explored using a toll-like receptor-7 agonist-induced rat model of HIC. The von Frey test was performed to assess bladder pain. Three days after HIC induction, the bladder was removed, and the expression of oxidative stress parameters in the bladder wall was investigated (reactive oxygen metabolites (ROMs), ·OH, and 8-hydroxy-2'-deoxyguanosine (8-OhdG)). : YKS had a ·OH scavenging capacity according to the ESR study. In the von Frey test, a significant decrease in the withdrawal threshold was observed in the HIC group compared with the control group; however, the decrease was ameliorated by the administration of YKS. Oxidative stress parameters showed increasing tendencies (ROMs test and 8-OHdG) or a significant increase (·OH) in the HIC group compared with the control group; however, the increase was significantly suppressed by the administration of YKS. : These findings suggest that YKS is effective against HIC and that its antioxidant activity is involved in the mechanism of action.
日本汉方药一贯煎（YKS）具有镇痛作用，用于治疗各种疼痛疾病。本研究旨在通过 Hunner 型间质性膀胱炎（HIC）的实验性大鼠模型，探讨 YKS 对 HIC 的作用，并探讨其抗氧化活性及其作用机制。
通过电子自旋共振（ESR）测定 YKS 对羟自由基（·OH）的清除能力来评估其抗氧化能力。然后，使用 Toll 样受体 7 激动剂诱导的 HIC 大鼠模型探讨 YKS 给药的效果。使用 von Frey 试验评估膀胱疼痛。HIC 诱导后 3 天，取出膀胱，研究膀胱壁氧化应激参数的表达（活性氧代谢物（ROMs）、·OH 和 8-羟基-2'-脱氧鸟苷（8-OhdG））。
根据 ESR 研究，YKS 具有·OH 清除能力。在 von Frey 试验中，与对照组相比，HIC 组的退缩阈值明显降低；然而，YKS 的给药改善了这种情况。与对照组相比，HIC 组的氧化应激参数呈上升趋势（ROMs 试验和 8-OhdG）或显著增加（·OH）；然而，YKS 的给药显著抑制了这种增加。
这些发现表明 YKS 对 HIC 有效，其抗氧化活性参与了作用机制。
