Athanasakopoulou Zoi, Diezel Celia, Braun Sascha D, Sofia Marina, Giannakopoulos Alexios, Monecke Stefan, Gary Dominik, Krähmer Domenique, Chatzopoulos Dimitris C, Touloudi Antonia, Birtsas Periklis, Palli Matina, Georgakopoulos Giorgos, Spyrou Vassiliki, Petinaki Efthymia, Ehricht Ralf, Billinis Charalambos
Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Leibniz Institute of Photonic Technology (IPHT), 07745 Jena, Germany.
Microorganisms. 2022 Jun 14;10(6):1217. doi: 10.3390/microorganisms10061217.
Wild and feral birds are known to be involved in the maintenance and dissemination of clinically-important antimicrobial-resistant pathogens, such as extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae. The aim of our study was to evaluate the presence of ESBL- and carbapenemase-producing among wild and feral birds from Greece and to describe their antimicrobial resistance characteristics. In this context, fecal samples of 362 birds were collected and cultured. Subsequently, the antimicrobial resistance pheno- and geno-type of all the obtained isolates were determined. A total of 12 multidrug-resistant (MDR), ESBL-producing were recovered from eight different wild bird species. Eleven of these isolates carried a group gene alone or in combination with and one carried only . AmpC, fluoroquinolone, trimethoprim/sulfamethoxazole, aminoglycoside and macrolide resistance genes were also detected. Additionally, one carbapenemase-producing was identified, harboring along with a combination of additional resistance genes. This report describes the occurrence of ESBL- and carbapenemase-producing among wild avian species in Greece, emphasizing the importance of incorporating wild birds in the assessment of AMR circulation in non-clinical settings.
已知野生和野生化鸟类参与了临床上重要的耐抗菌性病原体的维持和传播，例如产超广谱β-内酰胺酶（ESBL）和碳青霉烯酶的肠杆菌科细菌。我们研究的目的是评估希腊野生和野生化鸟类中产ESBL和碳青霉烯酶细菌的存在情况，并描述它们的抗菌耐药特征。在此背景下，收集了362只鸟类的粪便样本并进行培养。随后，确定了所有获得的分离株的抗菌耐药表型和基因型。从8种不同的野生鸟类中总共分离出12株产ESBL的多重耐药（MDR）细菌。这些分离株中有11株单独携带或与其他基因组合携带blaCTX-M基因，1株仅携带blaTEM基因。还检测到了AmpC、氟喹诺酮、甲氧苄啶/磺胺甲恶唑、氨基糖苷和大环内酯类耐药基因。此外，鉴定出1株产碳青霉烯酶细菌，携带blaNDM-1基因以及其他耐药基因的组合。本报告描述了希腊野生鸟类中产ESBL和碳青霉烯酶细菌的情况，强调了在非临床环境中评估抗菌药物耐药性传播时纳入野生鸟类的重要性。
