Institute of Sustainable and Renewable Energy (ISuRE), University Malaysia Sarawak, Kota Samarahan 94300, Malaysia.
Faculty of Resource Science and Technology, University Malaysia Sarawak, Kota Samarahan 94300, Malaysia.
Molecules. 2022 Jun 15;27(12):3840. doi: 10.3390/molecules27123840.
In the present work, the influence of geographical location on the fatty acid profiles, antioxidant potential, as well as cytotoxicity of edible dabai fruit fractions (kernel, skin, and pulp) were analyzed. The fatty acid profiles were determined by Gas Chromatography (GC), and the antioxidant activity was quantified with free 2,2-diphenyl-1-picr/ylhdrazyl, while the cytotoxicity was assessed by the brine shrimp lethality test. The results showed that the samples from Sibu, Serian, and Kapit geographical locations had a high content of the saturated fatty acids, ranging from 46.63% to 53.31% in the three fractions. The highest mono-saturated fatty acids (MUFA) content was found in Sibu. Serian and Kapit kernel fractions MUFA, however, ranged from 21.2% to 45.91%. No fatty acid composition was detected in Bentong and Kanowit. The fatty acid composition and DPPH free radical scavenging antioxidant activity of dabai were statistically independent using a multivariate analysis in different localities in Malaysia. The skin fraction had a more appreciable antioxidant potential and toxicity level than the pulp and kernel fractions. The highest antioxidant activity (EC 198.76 ± 1.06 µg/mL) with an LC value of 1387.22 µg/mL was obtained from the Sibu skin fraction. Therefore, the fatty acid composition, antioxidant, as well as cytotoxicity analyses of the extracts from different localities indicated that "geographical location" remarkably influenced fatty acid composition, antioxidant activity, and toxicity.
本工作分析了地理位置对食用白果（核、皮、肉）各部位脂肪酸组成、抗氧化能力及细胞毒性的影响。采用气相色谱法（GC）测定脂肪酸组成，用自由 2,2-二苯基-1-苦基肼（DPPH）法测定抗氧化活性，用卤虫致死试验评估细胞毒性。结果表明，来自诗巫、泗里街和加帛三地样品的饱和脂肪酸含量较高，在三个部位的范围为 46.63%至 53.31%。在核仁部位，单不饱和脂肪酸（MUFA）含量最高的是诗巫。泗里街和加帛的 MUFA 含量在核仁部位范围为 21.2%至 45.91%。在文冬和宽柔，未检测到脂肪酸组成。采用多元分析，发现不同产地白果的脂肪酸组成和 DPPH 自由基清除抗氧化活性在统计学上是独立的。果皮部位的抗氧化能力和毒性水平均高于果肉和核仁部位。来自诗巫果皮部位的抗氧化活性最高（EC 198.76±1.06µg/mL），LC 值为 1387.22µg/mL。因此，不同产地提取物的脂肪酸组成、抗氧化及细胞毒性分析表明，“地理位置”显著影响脂肪酸组成、抗氧化活性和毒性。
